Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Flow cytometry [2]
- Other assay [6]
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- Product number
- 45-0198-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD19 Monoclonal Antibody (SJ25C1), PerCP-Cyanine5.5, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The SJ25C1 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express this molecule. CD19, along with CD21, CD81, Leu13, and MHC class II, form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. The SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes. Applications Reported: This SJ25C1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This SJ25C1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- SJ25C1
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4°C, store in dark, DO NOT FREEZE!
Submitted references Persistence of functional memory B cells recognizing SARS-CoV-2 variants despite loss of specific IgG.
Methotrexate-loaded nanoparticles ameliorate experimental model of autoimmune arthritis by regulating the balance of interleukin-17-producing T cells and regulatory T cells.
Progranulin prevents regulatory NK cell cytotoxicity against antiviral T cells.
Subsets of ILC3-ILC1-like cells generate a diversity spectrum of innate lymphoid cells in human mucosal tissues.
Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation.
The Influence of Cytomegalovirus on Expression of HLA-G and its Ligand KIR2DL4 by Human Peripheral Blood Leucocyte Subsets.
Malaria-associated atypical memory B cells exhibit markedly reduced B cell receptor signaling and effector function.
Exposure-dependent control of malaria-induced inflammation in children.
Long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.
Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.
B cell analysis of ethnic groups in Mali with differential susceptibility to malaria.
Winklmeier S, Eisenhut K, Taskin D, Rübsamen H, Gerhards R, Schneider C, Wratil PR, Stern M, Eichhorn P, Keppler OT, Klein M, Mader S, Kümpfel T, Meinl E
iScience 2022 Jan 21;25(1):103659
iScience 2022 Jan 21;25(1):103659
Methotrexate-loaded nanoparticles ameliorate experimental model of autoimmune arthritis by regulating the balance of interleukin-17-producing T cells and regulatory T cells.
Park JS, Lee D, Yang S, Jeong HY, Na HS, Cho KH, Choi J, Koo H, Cho ML, Park SH
Journal of translational medicine 2022 Feb 11;20(1):85
Journal of translational medicine 2022 Feb 11;20(1):85
Progranulin prevents regulatory NK cell cytotoxicity against antiviral T cells.
Huang A, Shinde PV, Huang J, Senff T, Xu HC, Margotta C, Häussinger D, Willnow TE, Zhang J, Pandyra AA, Timm J, Weggen S, Lang KS, Lang PA
JCI insight 2019 Sep 5;4(17)
JCI insight 2019 Sep 5;4(17)
Subsets of ILC3-ILC1-like cells generate a diversity spectrum of innate lymphoid cells in human mucosal tissues.
Cella M, Gamini R, Sécca C, Collins PL, Zhao S, Peng V, Robinette ML, Schettini J, Zaitsev K, Gordon W, Bando JK, Yomogida K, Cortez V, Fronick C, Fulton R, Lin LL, Gilfillan S, Flavell RA, Shan L, Artyomov MN, Bowman M, Oltz EM, Jelinsky SA, Colonna M
Nature immunology 2019 Aug;20(8):980-991
Nature immunology 2019 Aug;20(8):980-991
Intracellular BH3 Profiling Reveals Shifts in Antiapoptotic Dependency in Human B Cell Maturation and Mitogen-Stimulated Proliferation.
Dai J, Luftig MA
Journal of immunology (Baltimore, Md. : 1950) 2018 Mar 1;200(5):1727-1736
Journal of immunology (Baltimore, Md. : 1950) 2018 Mar 1;200(5):1727-1736
The Influence of Cytomegalovirus on Expression of HLA-G and its Ligand KIR2DL4 by Human Peripheral Blood Leucocyte Subsets.
Albayati Z, Alyami A, Alomar S, Middleton D, Bonnett L, Aleem S, Flanagan BF, Christmas SE
Scandinavian journal of immunology 2017 Nov;86(5):396-407
Scandinavian journal of immunology 2017 Nov;86(5):396-407
Malaria-associated atypical memory B cells exhibit markedly reduced B cell receptor signaling and effector function.
Portugal S, Tipton CM, Sohn H, Kone Y, Wang J, Li S, Skinner J, Virtaneva K, Sturdevant DE, Porcella SF, Doumbo OK, Doumbo S, Kayentao K, Ongoiba A, Traore B, Sanz I, Pierce SK, Crompton PD
eLife 2015 May 8;4
eLife 2015 May 8;4
Exposure-dependent control of malaria-induced inflammation in children.
Portugal S, Moebius J, Skinner J, Doumbo S, Doumtabe D, Kone Y, Dia S, Kanakabandi K, Sturdevant DE, Virtaneva K, Porcella SF, Li S, Doumbo OK, Kayentao K, Ongoiba A, Traore B, Crompton PD
PLoS pathogens 2014 Apr;10(4):e1004079
PLoS pathogens 2014 Apr;10(4):e1004079
Long-lived Plasmodium falciparum specific memory B cells in naturally exposed Swedish travelers.
Ndungu FM, Lundblom K, Rono J, Illingworth J, Eriksson S, Färnert A
European journal of immunology 2013 Nov;43(11):2919-29
European journal of immunology 2013 Nov;43(11):2919-29
Chronic exposure to Plasmodium falciparum is associated with phenotypic evidence of B and T cell exhaustion.
Illingworth J, Butler NS, Roetynck S, Mwacharo J, Pierce SK, Bejon P, Crompton PD, Marsh K, Ndungu FM
Journal of immunology (Baltimore, Md. : 1950) 2013 Feb 1;190(3):1038-47
Journal of immunology (Baltimore, Md. : 1950) 2013 Feb 1;190(3):1038-47
B cell analysis of ethnic groups in Mali with differential susceptibility to malaria.
Portugal S, Doumtabe D, Traore B, Miller LH, Troye-Blomberg M, Doumbo OK, Dolo A, Pierce SK, Crompton PD
Malaria journal 2012 May 11;11:162
Malaria journal 2012 May 11;11:162
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0037-42) and Mouse IgG1 K Isotype Control PerCP-Cyanine5-5 (Product # 45-4714-82) (left) or Anti-Human CD19 PerCP-Cyanine5-5 (right). Cells in the lymphocyte gate were used for analysis.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0037-42) and Mouse IgG1 K Isotype Control PerCP-Cyanine5-5 (Product # 45-4714-82) (left) or Anti-Human CD19 PerCP-Cyanine5-5 (right). Cells in the lymphocyte gate were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL