Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [2]
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Validation data
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- Product number
- 10-4075 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-Human CD19 Antibody
- Antibody type
- Monoclonal
- Description
- CD19 is a B-cell specific cell-surface molecule of the Ig superfamily expressed by early pre-B cells in humans and mice until plasma cell differentiation. It plays a crucial role in mature B cell development as best exemplified by the finding that CD19 deficient mice have severely reduced mature B cell compartments. CD19 is specifically expressed in normal and neoplastic lymphoid cells. Human CD19 and mouse CD19 are functionally equivalent in vivo. Too high CD19 expression might result into too strong BCR signaling in the bone marrow and therefore causing negative selection. Too low CD19 expression might result into too little BCR signaling and thereby preventing the B cells to enter the mature pool (absence of positive selection). CD19 functions as the dominant signaling component of a multimolecular complex on the surface of mature B cells, alongside complement receptor CD21, and the tetraspanin membrane protein CD81 (TAPA-1), as well as CD225. CD19 plays a critical role in maintaining the balance between humoral, antigen-induced response and tolerance induction.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Intact normal human B cell cells we
re used as immunogen for this antib
ody.- Isotype
- IgG
- Antibody clone number
- CB19
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of hCD19. Anti-hCD19 antibody (Clone: CB19) was used at 2 µg/ml on Daudi lysate.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Surface flow cytometric analysis of CD19 on Raji cells using 0.5 µg antibody per 10^6 cells of antibody (Clone: CB19). Green represents isotype control; red represents anti-CD19 antibody (10-4075). Goat anti-mouse PE conjugate secondary antibody was used.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Cell Surface flow staining of hCD19 in PBMCs (lymphocytes gated) using 0.5 µg antibody per 10^6 cells of antibody (Clone: CB19). Green represents isotype control (ABEOMICS); red represents anti-hCD19 antibody (10-4075). Goat anti-mouse PE conjugate secondary antibody was used.
- Protocol
- Protocol