Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [4]
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Validation data
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- Product number
- NBP1-28375 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-28375, RRID:AB_1852927
- Product name
- Mouse Monoclonal CD19 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.1 mg/ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: CD19 Antibody (SJ25-C1) [NBP1-28375] - CD19 (red) stained in human skin, counterstained with DAPI (blue) at 20x. Tissue fixed with 4% PFA, permeabilized with 0.5% Triton, and blocked with 37% Egg DI, 5% Milk DI, and 3% FSG 2% BSA TBS Ca/Azide in that order. Anti-CD19 applied at 1:500 v/v, 4C, O/N. Alexa Fluor 568 secondary antibody, with DAPI counterstain. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD19 Antibody (SJ25-C1) [NBP1-28375] - Analysis using the Allophycocyanin conjugate of NBP1-28375. Multiple staining of peripheral blood lymphocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD19 Antibody (SJ25-C1) [NBP1-28375] - Analysis using the PE conjugate of NBP1-28375. Multiple staining of peripheral blood lymphocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD19 Antibody (SJ25-C1) [NBP1-28375] - Analysis of PerCP/Cy5.5 conjugate of NBP1-28375. Staining of normal human peripheral blood cells with Anti-Human CD3 FITC and Mouse IgG1 K Isotype Control PerCP-Cy5.5 (left) or Anti-Human CD19 PerCP-Cy5.5 (right). Cells in the lymphocyte gate were used fo
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD19 Antibody (SJ25-C1) [NBP1-28375] - Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19-APC and Mouse Anti-Human CD3-FITC.