MA5-15741

antibody from Invitrogen Antibodies

Targeting: CD19

Western blot

ELISA

Antibody data

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Validation data

Product number: MA5-15741 - Provider product page
Provider: Invitrogen Antibodies
Product name: CD19 Monoclonal Antibody (2E2B6B10)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-15741 targets CD19 in indirect ELISA, IHC and WB applications and shows reactivity with Human samples. The MA5-15741 immunogen is purified recombinant fragment of human CD19 expressed in E. Coli.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 2E2B6B10
Vial size: 100 μg
Concentration: Conc. Not Determined
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

CD19 protein structure - MA5-15741 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of B-lymphocyte antigen CD19 was performed using 70% confluent log phase Ramos cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD19 Monoclonal Antibody (2E2B6B10) (Product # MA5-15741) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing membrane as well as cytoplasmic localization. Panel e represents Jurkat cells having no expression of CD19. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. Immunofluorescence analysis of CD19 antibody was observed to be high in Ramos in comparison to low or negative expression in Jurkat . (doi :10.18632/oncotarget.24902).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of B-lymphocyte antigen CD19 was performed using 70% confluent log phase Ramos cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD19 Monoclonal Antibody (2E2B6B10) (Product # MA5-15741) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing membrane as well as cytoplasmic localization. Panel e represents Jurkat cells having no expression of CD19. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. Immunofluorescence analysis of CD19 antibody was observed to be high in Ramos in comparison to low or negative expression in Jurkat . (doi :10.18632/oncotarget.24902).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of B-lymphocyte antigen CD19 was performed using 70% confluent log phase Ramos cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD19 Monoclonal Antibody (2E2B6B10) (Product # MA5-15741) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing membrane as well as cytoplasmic localization. Panel e represents Jurkat cells having no expression of CD19. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. Immunofluorescence analysis of CD19 antibody was observed to be high in Ramos in comparison to low or negative expression in Jurkat . (doi :10.18632/oncotarget.24902).