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Immunohistochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
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- Product number
- MA5-16414 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD19 Monoclonal Antibody (SP110)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Heat-mediated antigen retrieval is recommended prior to staining, using an EDTA buffer, pH 8.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SP110
- Vial size
- 500 μL
- Concentration
- 0.05 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Role of the thymus in spontaneous development of a multi-organ autoimmune disease in human immune system mice.
Khosravi-Maharlooei M, Li H, Hoelzl M, Zhao G, Ruiz A, Misra A, Li Y, Teteloshvili N, Nauman G, Danzl N, Ding X, Pinker EY, Obradovic A, Yang YG, Iuga A, Creusot RJ, Winchester R, Sykes M
Journal of autoimmunity 2021 May;119:102612
Journal of autoimmunity 2021 May;119:102612
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical (paraffin) analysis of CD19 using anti-CD19 Monoclonal Antibody (Product # MA5-16414) in Tonsil Tissue. The recommended dilution for this antibody in immunohistochemistry applications is 1:200.
