PA5-27442

antibody from Invitrogen Antibodies

Targeting: CD19

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27442

Provider

Invitrogen Antibodies

Product name

CD19 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Raji, mouse leukocyte. Predicted reactivity: Pig (86%), Bovine (82%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.21 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CD19 using 30 µg Raji whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a CD19 polyclonal antibody (Product # PA5-27442) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CD19 was performed by separating 50 µg of Mouse tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CD19 Polyclonal Antibody (Product # PA5-27442) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CD19 Polyclonal Antibody (Product # PA5-27442). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD19 Polyclonal Antibody (Product # PA5-27442) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CD19 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CD19 Polyclonal Antibody (Product # PA5-27442) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of CD19 showing staining in the membrane of Raji cells. Raji cells were fixed in ice-cold MeOH for 5 min and stained using a CD19 polyclonal antibody (Product # PA5-27442) diluted at 1:1000. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CD19 Polyclonal Antibody detects CD19 protein at cell membrane by immunofluorescent analysis. Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CD19 stained by CD19 Polyclonal Antibody (Product # PA5-27442) diluted at 1:500. Blue: Fluoroshield with DAPI .

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of CD19 was performed in paraffin-embedded human tonsil tissue using Green: Dengue Virus Type 2 Envelope Monoclonal Antibody (GT643) (Product # MA5-17291) at a dilution of 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CD19 Polyclonal Antibody detects CD19 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human tonsil. CD19 stained by CD19 Polyclonal Antibody (Product # PA5-27442) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of CD3g was performed in mouse splenocytes cells fixed in 4% paraformaldehyde at 4°C for 15 min. The primary antibody used was CD3g Polyclonal Antibody (Product # PA5-29387) (red) at a dilution of 1:50. Black: Unlabelled sample was used as a control. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.