720237

antibody from Invitrogen Antibodies

Targeting: CYP2C9 CYP2C10, P450IIC9

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

720237

Provider

Invitrogen Antibodies

Product name

CYP2C9 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Tissue extracts of (30 µg lysate) Mouse liver (Lane 2), Rat liver (Lane 3), K-562 (Lane 4), HeLa (Lane 5), Jurkat (Lane 6) and Tissue extracts of (30 µg lysate) Mouse small intestine (Lane 7). The blots were probed with Anti-CYP2C9 Polyclonal Antibody (Product # 720237, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). Two isoforms of 56 and 18 kDa band corresponding to CYP2C9 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).