LS-C815498

antibody from LSBio

Targeting: TNFRSF4 ACT35, CD134, OX40, TXGP1L

Western blot (Enhanced validation)

Immunoprecipitation (Enhanced validation)

Antibody Data

Product number

LS-C815498

Provider

LSBio

Product name

TNFRSF4 / CD134 / OX40 Antibody (Preservative Free) LS-C815498

Antibody type

Polyclonal

Description

Protein A affinity chromatography

Reactivity

Rat

Host

Rabbit

Isotype

IgG

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-TNFRSF4 rabbit polyclonal antibody at 1:500 dilution. Lane A: MOLT4 Whole Cell Lysate. Lane B: THP1 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 47 kDa.

Immunoprecipitation

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

TNFRSF4 was immunoprecipitated using: Lane A: 0.5 mg THP-1 Whole Cell Lysate. Lane B: 0.5 mg A549 Whole Cell Lysate. Lane C:0.5 mg Hela Whole Cell Lysate. 4 uL anti-TNFRSF4 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-TNFRSF4 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 47 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

TNFRSF4 was immunoprecipitated using: Lane A: 0.5 mg THP-1 Whole Cell Lysate. Lane B: 0.5 mg A549 Whole Cell Lysate. Lane C:0.5 mg Hela Whole Cell Lysate. 4 uL anti-TNFRSF4 rabbit polyclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-TNFRSF4 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 29 kDa. Observed band size: 47 kDa.