Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB1436 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IGFALS/ALS Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IGFALS/ALS in direct ELISAs and Western blots.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P70389
- Isotype
- IgG
- Antibody clone number
- 199410
- Vial size
- 500 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The ternary IGF complex influences postnatal bone acquisition and the skeletal response to intermittent parathyroid hormone.
Yakar S, Bouxsein ML, Canalis E, Sun H, Glatt V, Gundberg C, Cohen P, Hwang D, Boisclair Y, Leroith D, Rosen CJ
The Journal of endocrinology 2006 May;189(2):289-99
The Journal of endocrinology 2006 May;189(2):289-99
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse IGFALS/ALS by Western Blot. Western blot shows mouse serum. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse IGFALS/ALS Monoclonal Antibody (Catalog # MAB1436) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for the ALS/IGFBP-3 Complex at approximately 140 kDa (as indicated) and ALS at approximately 80 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.