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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-59252 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ALS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: DCGCPLKALR DFALQNPSAV PRFVQAICEG DDCQPPAYTY NNITCASPPE VVGLDLRDLS EAHFAP Highest antigen sequence identity to the following orthologs: Mouse - 76%, Rat - 77%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Impaired kidney structure and function in spinal muscular atrophy.
Nery FC, Siranosian JJ, Rosales I, Deguise MO, Sharma A, Muhtaseb AW, Nwe P, Johnstone AJ, Zhang R, Fatouraei M, Huemer N, Alves CRR, Kothary R, Swoboda KJ
Neurology. Genetics 2019 Oct;5(5):e353
Neurology. Genetics 2019 Oct;5(5):e353
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ALS using ALS Polyclonal Antibody (Product # PA5-59252) (A) shows similar pattern to an independent ALS Polyclonal Antibody (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ALS in human cell line A-431 shows positivity in nucleus but excluded from the nucleoli. Samples were probed using an ALS Polyclonal Antibody (Product # PA5-59252).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ALS in human stomach using ALS Polyclonal Antibody (Product # PA5-59252) shows moderate cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ALS in human skeletal muscle using ALS Polyclonal Antibody (Product # PA5-59252) shows no positivity in myocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of ALS in human gallbladder using ALS Polyclonal Antibody (Product # PA5-59252) shows weak cytoplasmic positivity in glandular cells.
