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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- MA1-7621 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GSK3B Monoclonal Antibody (21A)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA1-7621 detects GSK3 Beta from human, rat, and Xenopus laevis samples.
- Antibody clone number
- 21A
- Concentration
- 1 mg/mL
Submitted references Soluble amyloid beta-protein dimers isolated from Alzheimer cortex directly induce Tau hyperphosphorylation and neuritic degeneration.
Jin M, Shepardson N, Yang T, Chen G, Walsh D, Selkoe DJ
Proceedings of the National Academy of Sciences of the United States of America 2011 Apr 5;108(14):5819-24
Proceedings of the National Academy of Sciences of the United States of America 2011 Apr 5;108(14):5819-24
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of GSK3 in MCF-7 cell extracts. Proteins were resolved by SDS-PAGE, transferred to a PVDF membrane and incubated with GSK-3 alpha/beta monoclonal antibody (Product # MA1-7621) at a dilution of 0.5 µg/mL. The signal was detected using a goat F (ab')2 anti-mouse IgG Alkaline Phosphatase conjugated antibody at a 1:5000 dilution and the membrane was incubated with CDP-substrate using the WesternStar™ method (Tropix). The membrane was then exposed to Kodak BioMax film.
