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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [7]
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- GTX111192 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111192, RRID:AB_1950461
- Product name
- GSK3 beta antibody [C1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references miR-214 targets the PTEN-mediated PI3K/Akt signaling pathway and regulates cell proliferation and apoptosis in ovarian cancer.
Antroquinonol, a Ubiquinone Derivative from the Mushroom Antrodia camphorata, Inhibits Colon Cancer Stem Cell-like Properties: Insights into the Molecular Mechanism and Inhibitory Targets.
PAK1 confers chemoresistance and poor outcome in non-small cell lung cancer via β-catenin-mediated stemness.
Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells.
γ-Tocotrienol reversal of epithelial-to-mesenchymal transition in human breast cancer cells is associated with inhibition of canonical Wnt signalling.
SH3BGRL3 Protein as a Potential Prognostic Biomarker for Urothelial Carcinoma: A Novel Binding Partner of Epidermal Growth Factor Receptor.
Radiation sensitization of tumor cells induced by shear stress: the roles of integrins and FAK.
Liu J, Chen W, Zhang H, Liu T, Zhao L
Oncology letters 2017 Nov;14(5):5711-5718
Oncology letters 2017 Nov;14(5):5711-5718
Antroquinonol, a Ubiquinone Derivative from the Mushroom Antrodia camphorata, Inhibits Colon Cancer Stem Cell-like Properties: Insights into the Molecular Mechanism and Inhibitory Targets.
Lin HC, Lin MH, Liao JH, Wu TH, Lee TH, Mi FL, Wu CH, Chen KC, Cheng CH, Lin CW
Journal of agricultural and food chemistry 2017 Jan 11;65(1):51-59
Journal of agricultural and food chemistry 2017 Jan 11;65(1):51-59
PAK1 confers chemoresistance and poor outcome in non-small cell lung cancer via β-catenin-mediated stemness.
Chen MJ, Wu DW, Wang YC, Chen CY, Lee H
Scientific reports 2016 Oct 7;6:34933
Scientific reports 2016 Oct 7;6:34933
Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells.
Xu YM, Wu DD, Zheng W, Yu FY, Yang F, Yao Y, Zhou Y, Ching YP, Lau AT
Oncotarget 2016 Feb 2;7(5):6146-58
Oncotarget 2016 Feb 2;7(5):6146-58
γ-Tocotrienol reversal of epithelial-to-mesenchymal transition in human breast cancer cells is associated with inhibition of canonical Wnt signalling.
Ahmed RA, Alawin OA, Sylvester PW
Cell proliferation 2016 Aug;49(4):460-70
Cell proliferation 2016 Aug;49(4):460-70
SH3BGRL3 Protein as a Potential Prognostic Biomarker for Urothelial Carcinoma: A Novel Binding Partner of Epidermal Growth Factor Receptor.
Chiang CY, Pan CC, Chang HY, Lai MD, Tzai TS, Tsai YS, Ling P, Liu HS, Lee BF, Cheng HL, Ho CL, Chen SH, Chow NH
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Dec 15;21(24):5601-11
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Dec 15;21(24):5601-11
Radiation sensitization of tumor cells induced by shear stress: the roles of integrins and FAK.
Luo CW, Wu CC, Ch'ang HJ
Biochimica et biophysica acta 2014 Sep;1843(9):2129-37
Biochimica et biophysica acta 2014 Sep;1843(9):2129-37
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: Raji 10% SDS PAGE GTX111192 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ?g of whole cell lysate) A: mouse brain 10% SDS PAGE GTX111192 diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GSK3 beta antibody [C1C3] detects GSK3 beta protein at cytoplasm by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GSK3 beta protein stained by GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:400.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H661 xenograft, using GSK3 beta(GTX111192) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GSK3 beta antibody [C1C3] detects GSK3 beta protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:500.
