Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-36646 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-GSK3 alpha/beta (Tyr279, Tyr216) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 46 and 51 kDa.
- Concentration
- 1 mg/mL
Submitted references GSK3β Inhibition Is the Molecular Pivot That Underlies the Mir-210-Induced Attenuation of Intrinsic Apoptosis Cascade during Hypoxia.
Marwarha G, Røsand Ø, Slagsvold KH, Høydal MA
International journal of molecular sciences 2022 Aug 19;23(16)
International journal of molecular sciences 2022 Aug 19;23(16)
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-GSK3 alpha/beta (Tyr279, Tyr216) in Lane 1: L02 whole cell lysate, Lane 2: L02 treated with PBS (1×PBS,PH7.4) for 30min whole cell lysate, Lane 3: L02 treated with PBS (1×PBS,PH7.4) for 60min whole cell lysate, Lane 4: L02 treated with PBS (1×PBS,PH7.4) for 120min whole cell lysate, Lane 5: L02 treated with PBS (1×PBS,PH7.4) for 180min whole cell lysate, Lane 6: L02 treated with PBS (1×PBS,PH7.4) for 240min whole cell lysate, Lane 7: C6 whole cell lysate. Samples were incubated with Phospho-GSK3 alpha/beta (Tyr279, Tyr216) polyclonal antibody (Product # PA5-36646) at a dilution of 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-GSK3 alpha/beta (Tyr279, Tyr216) in Lane 1: L02 whole cell lysate, Lane 2: L02 treated with PBS (1×PBS,PH7.4) for 30min whole cell lysate, Lane 3: L02 treated with PBS (1×PBS,PH7.4) for 60min whole cell lysate, Lane 4: L02 treated with PBS (1×PBS,PH7.4) for 120min whole cell lysate, Lane 5: L02 treated with PBS (1×PBS,PH7.4) for 180min whole cell lysate, Lane 6: L02 treated with PBS (1×PBS,PH7.4) for 240min whole cell lysate, Lane 7: C6 whole cell lysate. Samples were incubated with Phospho-GSK3 alpha/beta (Tyr279, Tyr216) polyclonal antibody (Product # PA5-36646) at a dilution of 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-GSK3 alpha/beta (Tyr279, Tyr216) in paraffin-embedded human breast carcinoma tissue. Samples were incubated with Phospho-GSK3 alpha/beta (Tyr279, Tyr216) polyclonal antibody (Product # PA5-36646) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 miR-210 attenuates the hypoxia-induced increase in GSK3beta kinase activity. ( A - C ) Representative Western blots ( A ) and quantitative densitometric analysis determining the inhibitory phosphorylation of GSK3beta at the Ser 9 residue ( B ) concomitant with the activating phosphorylation of GSK3beta at the Tyr 216 residue ( C ). ( D ) Quantitative GSK3beta kinase activity assay in native non-denatured lysates. miR-210 expression levels in the corresponding respective cell lysates were determined by the miR-210 hybridization immunoassay (as described in Section 4.3 ). Data from the Western blot and densitometric analysis are expressed as mean fold-change +- S.D from three biological replicates belonging to each experimental group ( n = 3). Data from the GSK3beta activity assay are expressed as experimental blank-corrected absorbances (O.D) measured at lambda 405 (405 nm). Data from the GSK3beta activity assay are expressed as mean +- S.D from three technical replicates for each of the four biological replicates belonging to each experimental group ( n = 4). miR-210 expression levels are depicted as fold-change +- S.D. * p
