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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AM00066PU-N - Provider product page
- Provider
- OriGene
- Product name
- GSK3 beta (GSK3B) (N-term) (incl. pos. control) mouse monoclonal antibody, clone 11B9, Purified
- Antibody type
- Monoclonal
- Description
- GSK3 beta (GSK3B) (N-term) (incl. pos. control) mouse monoclonal antibody, clone 11B9, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- GSK3B
- Isotype
- IgG
- Antibody clone number
- 11B9
- Vial size
- 100 µg
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Supportive validation
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- OriGene (provider)
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- Experimental details
- Detection of Endogenous GSK3 beta: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab GSK3-11B9 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2:SW480 Lane 3: SW620 Lane 4: HT29 Lane 5: MCF7 Lane 6: MDA-MB-231 Lane 7: T47D
- Validation comment
- WB
