Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB0479 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0479, RRID:AB_982602
- Product name
- GSK3B (phospho S21/29) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of GSK3B.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Glycogen synthase kinase-3beta activity is required for androgen-stimulated gene expression in prostate cancer.
14-3-3 binds to and mediates phosphorylation of microtubule-associated tau protein by Ser9-phosphorylated glycogen synthase kinase 3beta in the brain.
Suppression of androgen receptor-mediated transactivation and cell growth by the glycogen synthase kinase 3 beta in prostate cells.
Liao X, Thrasher JB, Holzbeierlein J, Stanley S, Li B
Endocrinology 2004 Jun;145(6):2941-9
Endocrinology 2004 Jun;145(6):2941-9
14-3-3 binds to and mediates phosphorylation of microtubule-associated tau protein by Ser9-phosphorylated glycogen synthase kinase 3beta in the brain.
Yuan Z, Agarwal-Mawal A, Paudel HK
The Journal of biological chemistry 2004 Jun 18;279(25):26105-14
The Journal of biological chemistry 2004 Jun 18;279(25):26105-14
Suppression of androgen receptor-mediated transactivation and cell growth by the glycogen synthase kinase 3 beta in prostate cells.
Wang L, Lin HK, Hu YC, Xie S, Yang L, Chang C
The Journal of biological chemistry 2004 Jul 30;279(31):32444-52
The Journal of biological chemistry 2004 Jul 30;279(31):32444-52
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The GSK3B (phospho S21/29) polyclonal antibody (Cat # PAB0479) is used in Western blot to detect Phospho-GSK3B-S21/29 in mouse thymus tissue lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with GSK3B (phospho S21/29) polyclonal antibody (Cat # PAB0479) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
