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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- MAB1087 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB1087, RRID:AB_1575329
- Product name
- GSK3B monoclonal antibody, clone 1F7
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant GSK3B.
- Isotype
- IgG
- Antibody clone number
- 1F7
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references In vivo regulation of GSK3 phosphorylation by cholinergic and NMDA receptors.
Structural basis for recruitment of glycogen synthase kinase 3beta to the axin-APC scaffold complex.
Direct, activating interaction between glycogen synthase kinase-3beta and p53 after DNA damage.
De Sarno P, Bijur GN, Zmijewska AA, Li X, Jope RS
Neurobiology of aging 2006 Mar;27(3):413-22
Neurobiology of aging 2006 Mar;27(3):413-22
Structural basis for recruitment of glycogen synthase kinase 3beta to the axin-APC scaffold complex.
Dajani R, Fraser E, Roe SM, Yeo M, Good VM, Thompson V, Dale TC, Pearl LH
The EMBO journal 2003 Feb 3;22(3):494-501
The EMBO journal 2003 Feb 3;22(3):494-501
Direct, activating interaction between glycogen synthase kinase-3beta and p53 after DNA damage.
Watcharasit P, Bijur GN, Zmijewski JW, Song L, Zmijewska A, Chen X, Johnson GV, Jope RS
Proceedings of the National Academy of Sciences of the United States of America 2002 Jun 11;99(12):7951-5
Proceedings of the National Academy of Sciences of the United States of America 2002 Jun 11;99(12):7951-5
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- Experimental details
- Western blot analysis of HeLa cell lysate (each 30 ug) were resolved by SDS - PAGE , transferred to PVDF membrane and probed with GSK3B monoclonal antibody , clone 1F7 (1 : 2000) (Cat # MAB1087). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
