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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB10055 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10055, RRID:AB_1708179
- Product name
- GSK3B (phospho S9) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of GSK3B.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Crystal structure of glycogen synthase kinase 3 beta: structural basis for phosphate-primed substrate specificity and autoinhibition.
The structure of phosphorylated GSK-3beta complexed with a peptide, FRATtide, that inhibits beta-catenin phosphorylation.
Cloning and characterization of a novel human ninein protein that interacts with the glycogen synthase kinase 3beta.
Dajani R, Fraser E, Roe SM, Young N, Good V, Dale TC, Pearl LH
Cell 2001 Jun 15;105(6):721-32
Cell 2001 Jun 15;105(6):721-32
The structure of phosphorylated GSK-3beta complexed with a peptide, FRATtide, that inhibits beta-catenin phosphorylation.
Bax B, Carter PS, Lewis C, Guy AR, Bridges A, Tanner R, Pettman G, Mannix C, Culbert AA, Brown MJ, Smith DG, Reith AD
Structure (London, England : 1993) 2001 Dec;9(12):1143-52
Structure (London, England : 1993) 2001 Dec;9(12):1143-52
Cloning and characterization of a novel human ninein protein that interacts with the glycogen synthase kinase 3beta.
Hong YR, Chen CH, Chang JH, Wang S, Sy WD, Chou CK, Howng SL
Biochimica et biophysica acta 2000 Jul 24;1492(2-3):513-6
Biochimica et biophysica acta 2000 Jul 24;1492(2-3):513-6
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using GSK3B (phospho S9) polyclonal antibody (Cat # PAB10055) at a 1:1,000 dilution.All lanes contain human 293T whole cell lysate showing a band at 47 KDa.Cells were serum starved for 24 h prior to extraction.Key : Lane 1, Control; Lane 2, treated with IGF-1 (100 ng/mL) for 20'; lane 3, pre-treated with 10 uM selective PI3K inhibitor and treated with IGF-1 (100 ng/mL) for 20'.Molecular weight markers confirm a MW of ~ 49 KDa.Use a 1 : 2,000 dilution of HRP Goat-a-Rabbit IgG for detection.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry of GSK3B (phospho S9) polyclonal antibody (Cat # PAB10055) was used at a 1 : 200 dilution to detect GSK3B by immunohistochemistry in human ovarian cancer tumor tissue.Tissue was formalin-fixed and paraffin embedded.Personal Communication, Alan Yen, Life Span Biosciences, Seattle, WA.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
