Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- P00791-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Phospho-GSK3 beta (Ser9) Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for GSK 3 BETA/GSK3B detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, IHC, ICC, WB. Reactive species: Human GSK 3 BETA/GSK3B information: Molecular Weight: 46744 MW; Subcellular Localization: Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosphorylated form to the cell membrane; Tissue Specificity: Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. Colocalizes with EIF2AK2/PKR and TAU in the Alzheimer disease (AD) brain.
- Reactivity
- Human
- Host
- Rabbit
- Antibody clone number
- IFC-7
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
Submitted references Water extract of cacumen platycladi promotes hair growth through the Akt/GSK3β/β-catenin signaling pathway.
Comprehensive circular RNA profiling reveals the regulatory role of circRNA_0007694 in papillary thyroid carcinoma.
Downregulation of cytokeratin 18 induces cellular partial EMT and stemness through increasing EpCAM expression in breast cancer.
Essential roles of Akt/Snail pathway in microcystin-LR-induced tight junction toxicity in Sertoli cell.
Epicatechin Plus Treadmill Exercise are Neuroprotective Against Moderate-stage Amyloid Precursor Protein/Presenilin 1 Mice.
Vibration exercise decreases insulin resistance and modulates the insulin signaling pathway in a type 2 diabetic rat model.
Fu H, Li W, Weng Z, Huang Z, Liu J, Mao Q, Ding B
Frontiers in pharmacology 2023;14:1038039
Frontiers in pharmacology 2023;14:1038039
Comprehensive circular RNA profiling reveals the regulatory role of circRNA_0007694 in papillary thyroid carcinoma.
Long MY, Chen JW, Zhu Y, Luo DY, Lin SJ, Peng XZ, Tan LP, Li HH
American journal of translational research 2020;12(4):1362-1378
American journal of translational research 2020;12(4):1362-1378
Downregulation of cytokeratin 18 induces cellular partial EMT and stemness through increasing EpCAM expression in breast cancer.
Shi R, Liu L, Wang F, He Y, Niu Y, Wang C, Zhang X, Zhang X, Zhang H, Chen M, Wang Y
Cellular signalling 2020 Dec;76:109810
Cellular signalling 2020 Dec;76:109810
Essential roles of Akt/Snail pathway in microcystin-LR-induced tight junction toxicity in Sertoli cell.
Zhou Y, Geng X, Chen Y, Shi H, Yang Y, Zhu C, Yu G, Tang Z
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2018 Feb;112:290-298
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2018 Feb;112:290-298
Epicatechin Plus Treadmill Exercise are Neuroprotective Against Moderate-stage Amyloid Precursor Protein/Presenilin 1 Mice.
Zhang Z, Wu H, Huang H
Pharmacognosy magazine 2016 May;12(Suppl 2):S139-46
Pharmacognosy magazine 2016 May;12(Suppl 2):S139-46
Vibration exercise decreases insulin resistance and modulates the insulin signaling pathway in a type 2 diabetic rat model.
Liu Y, Liu C, Lu ML, Tang FT, Hou XW, Yang J, Liu T
International journal of clinical and experimental medicine 2015;8(8):13136-44
International journal of clinical and experimental medicine 2015;8(8):13136-44
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human kidney, using Phospho-GSK3 beta (Ser9) Antibody(P00791-1)GSK3B was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GSK3B Antibody (P00791-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image