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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [12]
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- Validations
- Immunohistochemistry [1]
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- Product number
- M00791 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-GSK3 beta Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for GSK 3 BETA/GSK3B detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat GSK 3 BETA/GSK3B information: Molecular Weight: 46903 MW; Subcellular Localization: Cytoplasm . Nucleus . Cell membrane ; Tissue Specificity: Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. Colocalizes with EIF2AK2/PKR and TAU in the Alzheimer disease (AD) brain.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Antibody clone number
- EB-7
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
Submitted references Water extract of cacumen platycladi promotes hair growth through the Akt/GSK3β/β-catenin signaling pathway.
Cerebroprotein hydrolysate injection is involved in promoting long-term angiogenesis, vessel diameter and density after cerebral ischemia in mice.
Anti-Myocardial Ischemia Reperfusion Injury Mechanism of Dried Ginger-Aconite Decoction Based on Network Pharmacology.
Comprehensive circular RNA profiling reveals the regulatory role of circRNA_0007694 in papillary thyroid carcinoma.
Downregulation of cytokeratin 18 induces cellular partial EMT and stemness through increasing EpCAM expression in breast cancer.
The PI3K/Akt/GSK-3β/ROS/eIF2B pathway promotes breast cancer growth and metastasis via suppression of NK cell cytotoxicity and tumor cell susceptibility.
Artemisinin Ameliorates Osteoarthritis by Inhibiting the Wnt/β-Catenin Signaling Pathway.
Down-regulation of long non-coding RNA ESCCAL_1 inhibits tumor growth of esophageal squamous cell carcinoma in a xenograft mouse model.
Essential roles of Akt/Snail pathway in microcystin-LR-induced tight junction toxicity in Sertoli cell.
Analysis of the effect of rutin on GSK-3β and TNF-α expression in lung cancer.
Stereotactic injection of shrna GSK-3β-AAV promotes axonal regeneration after spinal cord injury.
Protection of ghrelin postconditioning on hypoxia/reoxygenation in gastric epithelial cells.
Fu H, Li W, Weng Z, Huang Z, Liu J, Mao Q, Ding B
Frontiers in pharmacology 2023;14:1038039
Frontiers in pharmacology 2023;14:1038039
Cerebroprotein hydrolysate injection is involved in promoting long-term angiogenesis, vessel diameter and density after cerebral ischemia in mice.
Myagmar BO, Chen R, Zhang X, Xu R, Jiang W, Cao W, Ji H, Zhang X
Life sciences 2022 Jul 1;300:120568
Life sciences 2022 Jul 1;300:120568
Anti-Myocardial Ischemia Reperfusion Injury Mechanism of Dried Ginger-Aconite Decoction Based on Network Pharmacology.
Xie F, Wu YY, Duan GJ, Wang B, Gao F, Wei PF, Chen L, Liu AP, Li M
Frontiers in pharmacology 2021;12:609702
Frontiers in pharmacology 2021;12:609702
Comprehensive circular RNA profiling reveals the regulatory role of circRNA_0007694 in papillary thyroid carcinoma.
Long MY, Chen JW, Zhu Y, Luo DY, Lin SJ, Peng XZ, Tan LP, Li HH
American journal of translational research 2020;12(4):1362-1378
American journal of translational research 2020;12(4):1362-1378
Downregulation of cytokeratin 18 induces cellular partial EMT and stemness through increasing EpCAM expression in breast cancer.
Shi R, Liu L, Wang F, He Y, Niu Y, Wang C, Zhang X, Zhang X, Zhang H, Chen M, Wang Y
Cellular signalling 2020 Dec;76:109810
Cellular signalling 2020 Dec;76:109810
The PI3K/Akt/GSK-3β/ROS/eIF2B pathway promotes breast cancer growth and metastasis via suppression of NK cell cytotoxicity and tumor cell susceptibility.
Jin F, Wu Z, Hu X, Zhang J, Gao Z, Han X, Qin J, Li C, Wang Y
Cancer biology & medicine 2019 Feb;16(1):38-54
Cancer biology & medicine 2019 Feb;16(1):38-54
Artemisinin Ameliorates Osteoarthritis by Inhibiting the Wnt/β-Catenin Signaling Pathway.
Zhong G, Liang R, Yao J, Li J, Jiang T, Liu J, Le Y, Shen C, Long H, Lu H, Ma S, Luo J, Miao Z, Su W, Zheng L, Zhao J
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;51(6):2575-2590
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;51(6):2575-2590
Down-regulation of long non-coding RNA ESCCAL_1 inhibits tumor growth of esophageal squamous cell carcinoma in a xenograft mouse model.
Cui Y, Wu W, Lv P, Zhang J, Bai B, Cao W
Oncotarget 2018 Jan 2;9(1):783-790
Oncotarget 2018 Jan 2;9(1):783-790
Essential roles of Akt/Snail pathway in microcystin-LR-induced tight junction toxicity in Sertoli cell.
Zhou Y, Geng X, Chen Y, Shi H, Yang Y, Zhu C, Yu G, Tang Z
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2018 Feb;112:290-298
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2018 Feb;112:290-298
Analysis of the effect of rutin on GSK-3β and TNF-α expression in lung cancer.
Wu F, Chen J, Fan LM, Liu K, Zhang N, Li SW, Zhu H, Gao HC
Experimental and therapeutic medicine 2017 Jul;14(1):127-130
Experimental and therapeutic medicine 2017 Jul;14(1):127-130
Stereotactic injection of shrna GSK-3β-AAV promotes axonal regeneration after spinal cord injury.
Zuo YC, Xiong NX, Zhao HY
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2016 Aug;36(4):548-553
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2016 Aug;36(4):548-553
Protection of ghrelin postconditioning on hypoxia/reoxygenation in gastric epithelial cells.
Liu ZB, Fei SJ, Zhu SP, Zhu JZ, Han HX, Dong QJ, Zhang JF
World journal of gastroenterology 2012 Oct 14;18(38):5377-88
World journal of gastroenterology 2012 Oct 14;18(38):5377-88
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinome, using GSK3 beta Antibody(M00791)GSK3B was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GSK3B Antibody (M00791)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image
