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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-29251 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GSK3B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, K562, Mouse brain. Predicted reactivity: Mouse (96%), Rat (89%), Zebrafish (83%), Xenopus laevis (82%), Pig (90%), Sheep (90%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.57 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GSK3 beta using 30 µg of Raji lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a GSK3 beta polyclonal antibody (Product # PA5-29251) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GSK3B was performed by separating 30 µg of whole cell extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a GSK3B Polyclonal Antibody (Product # PA5-29251) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GSK3B Polyclonal Antibody (Product # PA5-29251). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 10% SDS PAGE. GSK3B Polyclonal Antibody (Product # PA5-29251) diluted at 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GSK3B was performed in SK-N-SH cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GSK3B Polyclonal Antibody (Product # PA5-29251) diluted at 1:400. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of GSK3B was performed in paraffin-embedded rat brain tissue using GSK3B Polyclonal Antibody (Product # PA5-29251) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H661 xenograft, using GSK3 beta (Product # PA5-29251) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
