AM00066PU-N

antibody from Acris Antibodies GmbH

Targeting: GSK3B

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

AM00066PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM00066PU-N, RRID:AB_1001938

Product name

anti GSK3 beta (N-term) (incl. pos. control)

Antibody type

Monoclonal

Antigen

Synthetic phosphopeptide conjugated to KLH.

Reactivity

Human, Mouse, Rat, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

11B9

Vial size

0.1 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Detection of Endogenous GSK3 beta: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab GSK3-11B9 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431Lane 2:SW480 Lane 3: SW620 Lane 4: HT29 Lane 5: MCF7 Lane 6: MDA-MB-231 Lane 7: T47D