Antibody data
- Antibody Data
- Antigen structure
- References [24]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB2164-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CXCR2/IL-8RB Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Stains mouse CXCR2 transfected cells but not CXCR1 transfectants.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P35343
- Isotype
- IgG
- Antibody clone number
- 242216
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling.
Estrogen stimulates female cancer progression by inducing myeloid-derived suppressive cells: investigations on pregnant and non-pregnant experimental models.
Novel adherent CD11b+ Gr-1+ tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment.
The mTOR signal regulates myeloid-derived suppressor cells differentiation and immunosuppressive function in acute kidney injury.
Microbiome-mediated neutrophil recruitment via CXCR2 and protection from amebic colitis.
Sphingolipid pathway regulates innate immune responses at the fetomaternal interface during pregnancy.
The calcineurin-NFAT axis controls allograft immunity in myeloid-derived suppressor cells through reprogramming T cell differentiation.
CXCR2-mediated tumor-associated neutrophil recruitment is regulated by IFN-β.
mTOR limits the recruitment of CD11b+Gr1+Ly6Chigh myeloid-derived suppressor cells in protecting against murine immunological hepatic injury.
Fibrates ameliorate the course of bacterial sepsis by promoting neutrophil recruitment via CXCR2.
Platelets guide the formation of early metastatic niches.
IL-17A produced by neutrophils protects against pneumonic plague through orchestrating IFN-γ-activated macrophage programming.
Phosphatase Wip1 negatively regulates neutrophil migration and inflammation.
Dendritic cells: In vitro culture in two- and three-dimensional collagen systems and expression of collagen receptors in tumors and atherosclerotic microenvironments.
Inefficient antimicrobial functions of innate phagocytes render infant mice more susceptible to bacterial infection.
G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
Respiratory syncytial virus induces airway insensitivity to beta-agonists in BALB/c mice.
Neuroprotection and remyelination after autoimmune demyelination in mice that inducibly overexpress CXCL1.
Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
An age-associated increase in pulmonary inflammation after burn injury is abrogated by CXCR2 inhibition.
Chemokine-mediated rapid turnover of myeloid-derived suppressor cells in tumor-bearing mice.
HIF-1alpha regulates epithelial inflammation by cell autonomous NFkappaB activation and paracrine stromal remodeling.
Genetic removal of the A2A adenosine receptor enhances pulmonary inflammation, mucin production, and angiogenesis in adenosine deaminase-deficient mice.
An essential role for IL-17 in preventing pathogen-initiated bone destruction: recruitment of neutrophils to inflamed bone requires IL-17 receptor-dependent signals.
Capitano ML, Mor-Vaknin N, Saha AK, Cooper S, Legendre M, Guo H, Contreras-Galindo R, Kappes F, Sartor MA, Lee CT, Huang X, Markovitz DM, Broxmeyer HE
The Journal of clinical investigation 2019 May 20;129(6):2555-2570
The Journal of clinical investigation 2019 May 20;129(6):2555-2570
Estrogen stimulates female cancer progression by inducing myeloid-derived suppressive cells: investigations on pregnant and non-pregnant experimental models.
Kozasa K, Mabuchi S, Matsumoto Y, Kuroda H, Yokoi E, Komura N, Kawano M, Takahashi R, Sasano T, Shimura K, Kodama M, Hashimoto K, Sawada K, Nagasaka K, Kimura T
Oncotarget 2019 Mar 8;10(20):1887-1902
Oncotarget 2019 Mar 8;10(20):1887-1902
Novel adherent CD11b+ Gr-1+ tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment.
Tsubaki T, Kadonosono T, Sakurai S, Shiozawa T, Goto T, Sakai S, Kuchimaru T, Sakamoto T, Watanabe H, Kondoh G, Kizaka-Kondoh S
Oncotarget 2018 Feb 16;9(13):11209-11226
Oncotarget 2018 Feb 16;9(13):11209-11226
The mTOR signal regulates myeloid-derived suppressor cells differentiation and immunosuppressive function in acute kidney injury.
Zhang C, Wang S, Li J, Zhang W, Zheng L, Yang C, Zhu T, Rong R
Cell death & disease 2017 Mar 23;8(3):e2695
Cell death & disease 2017 Mar 23;8(3):e2695
Microbiome-mediated neutrophil recruitment via CXCR2 and protection from amebic colitis.
Watanabe K, Gilchrist CA, Uddin MJ, Burgess SL, Abhyankar MM, Moonah SN, Noor Z, Donowitz JR, Schneider BN, Arju T, Ahmed E, Kabir M, Alam M, Haque R, Pramoonjago P, Mehrad B, Petri WA Jr
PLoS pathogens 2017 Aug;13(8):e1006513
PLoS pathogens 2017 Aug;13(8):e1006513
Sphingolipid pathway regulates innate immune responses at the fetomaternal interface during pregnancy.
Mizugishi K, Inoue T, Hatayama H, Bielawski J, Pierce JS, Sato Y, Takaori-Kondo A, Konishi I, Yamashita K
The Journal of biological chemistry 2015 Jan 23;290(4):2053-68
The Journal of biological chemistry 2015 Jan 23;290(4):2053-68
The calcineurin-NFAT axis controls allograft immunity in myeloid-derived suppressor cells through reprogramming T cell differentiation.
Wang X, Bi Y, Xue L, Liao J, Chen X, Lu Y, Zhang Z, Wang J, Liu H, Yang H, Liu G
Molecular and cellular biology 2015 Feb;35(3):598-609
Molecular and cellular biology 2015 Feb;35(3):598-609
CXCR2-mediated tumor-associated neutrophil recruitment is regulated by IFN-β.
Jablonska J, Wu CF, Andzinski L, Leschner S, Weiss S
International journal of cancer 2014 Mar 15;134(6):1346-58
International journal of cancer 2014 Mar 15;134(6):1346-58
mTOR limits the recruitment of CD11b+Gr1+Ly6Chigh myeloid-derived suppressor cells in protecting against murine immunological hepatic injury.
Zhang Y, Bi Y, Yang H, Chen X, Liu H, Lu Y, Zhang Z, Liao J, Yang S, Chu Y, Yang R, Liu G
Journal of leukocyte biology 2014 Jun;95(6):961-70
Journal of leukocyte biology 2014 Jun;95(6):961-70
Fibrates ameliorate the course of bacterial sepsis by promoting neutrophil recruitment via CXCR2.
Tancevski I, Nairz M, Duwensee K, Auer K, Schroll A, Heim C, Feistritzer C, Hoefer J, Gerner RR, Moschen AR, Heller I, Pallweber P, Li X, Theurl M, Demetz E, Wolf AM, Wolf D, Eller P, Ritsch A, Weiss G
EMBO molecular medicine 2014 Jun;6(6):810-20
EMBO molecular medicine 2014 Jun;6(6):810-20
Platelets guide the formation of early metastatic niches.
Labelle M, Begum S, Hynes RO
Proceedings of the National Academy of Sciences of the United States of America 2014 Jul 29;111(30):E3053-61
Proceedings of the National Academy of Sciences of the United States of America 2014 Jul 29;111(30):E3053-61
IL-17A produced by neutrophils protects against pneumonic plague through orchestrating IFN-γ-activated macrophage programming.
Bi Y, Zhou J, Yang H, Wang X, Zhang X, Wang Q, Wu X, Han Y, Song Y, Tan Y, Du Z, Yang H, Zhou D, Cui Y, Zhou L, Yan Y, Zhang P, Guo Z, Wang X, Liu G, Yang R
Journal of immunology (Baltimore, Md. : 1950) 2014 Jan 15;192(2):704-13
Journal of immunology (Baltimore, Md. : 1950) 2014 Jan 15;192(2):704-13
Phosphatase Wip1 negatively regulates neutrophil migration and inflammation.
Sun B, Hu X, Liu G, Ma B, Xu Y, Yang T, Shi J, Yang F, Li H, Zhang L, Zhao Y
Journal of immunology (Baltimore, Md. : 1950) 2014 Feb 1;192(3):1184-95
Journal of immunology (Baltimore, Md. : 1950) 2014 Feb 1;192(3):1184-95
Dendritic cells: In vitro culture in two- and three-dimensional collagen systems and expression of collagen receptors in tumors and atherosclerotic microenvironments.
Sprague L, Muccioli M, Pate M, Singh M, Xiong C, Ostermann A, Niese B, Li Y, Li Y, Courreges MC, Benencia F
Experimental cell research 2014 Apr 15;323(1):7-27
Experimental cell research 2014 Apr 15;323(1):7-27
Inefficient antimicrobial functions of innate phagocytes render infant mice more susceptible to bacterial infection.
Zhang Q, Coveney AP, Yu S, Liu JH, Li Y, Blankson S, Redmond HP, Wang JH, Wang J
European journal of immunology 2013 May;43(5):1322-32
European journal of immunology 2013 May;43(5):1322-32
G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
Raghuwanshi SK, Smith N, Rivers EJ, Thomas AJ, Sutton N, Hu Y, Mukhopadhyay S, Chen XL, Leung T, Richardson RM
Journal of immunology (Baltimore, Md. : 1950) 2013 May 15;190(10):5329-36
Journal of immunology (Baltimore, Md. : 1950) 2013 May 15;190(10):5329-36
Respiratory syncytial virus induces airway insensitivity to beta-agonists in BALB/c mice.
Traylor ZP, Yu EN, Davis IC
American journal of physiology. Lung cellular and molecular physiology 2010 Mar;298(3):L437-45
American journal of physiology. Lung cellular and molecular physiology 2010 Mar;298(3):L437-45
Neuroprotection and remyelination after autoimmune demyelination in mice that inducibly overexpress CXCL1.
Omari KM, Lutz SE, Santambrogio L, Lira SA, Raine CS
The American journal of pathology 2009 Jan;174(1):164-76
The American journal of pathology 2009 Jan;174(1):164-76
Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA Jr, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ
Proceedings of the National Academy of Sciences of the United States of America 2009 Apr 7;106(14):5954-9
Proceedings of the National Academy of Sciences of the United States of America 2009 Apr 7;106(14):5954-9
An age-associated increase in pulmonary inflammation after burn injury is abrogated by CXCR2 inhibition.
Nomellini V, Faunce DE, Gomez CR, Kovacs EJ
Journal of leukocyte biology 2008 Jun;83(6):1493-501
Journal of leukocyte biology 2008 Jun;83(6):1493-501
Chemokine-mediated rapid turnover of myeloid-derived suppressor cells in tumor-bearing mice.
Sawanobori Y, Ueha S, Kurachi M, Shimaoka T, Talmadge JE, Abe J, Shono Y, Kitabatake M, Kakimi K, Mukaida N, Matsushima K
Blood 2008 Jun 15;111(12):5457-66
Blood 2008 Jun 15;111(12):5457-66
HIF-1alpha regulates epithelial inflammation by cell autonomous NFkappaB activation and paracrine stromal remodeling.
Scortegagna M, Cataisson C, Martin RJ, Hicklin DJ, Schreiber RD, Yuspa SH, Arbeit JM
Blood 2008 Apr 1;111(7):3343-54
Blood 2008 Apr 1;111(7):3343-54
Genetic removal of the A2A adenosine receptor enhances pulmonary inflammation, mucin production, and angiogenesis in adenosine deaminase-deficient mice.
Mohsenin A, Mi T, Xia Y, Kellems RE, Chen JF, Blackburn MR
American journal of physiology. Lung cellular and molecular physiology 2007 Sep;293(3):L753-61
American journal of physiology. Lung cellular and molecular physiology 2007 Sep;293(3):L753-61
An essential role for IL-17 in preventing pathogen-initiated bone destruction: recruitment of neutrophils to inflamed bone requires IL-17 receptor-dependent signals.
Yu JJ, Ruddy MJ, Wong GC, Sfintescu C, Baker PJ, Smith JB, Evans RT, Gaffen SL
Blood 2007 May 1;109(9):3794-802
Blood 2007 May 1;109(9):3794-802
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CXCR2/IL-8 RB in Mouse Spleen. CXCR2/IL-8 RB was detected in perfusion fixed frozen sections of mouse spleen using Rat Anti-Mouse CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB2164) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell sufaces in splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CXCR2/IL-8 RB in HEK293 Human Cell Line Transfected with Mouse CXCR2/IL-8 RB and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse CXCR2/IL-8 RB or (B) mouse CXCR1/IL-8 RA and eGFP was stained with Rat Anti-Mouse CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB2164) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB006).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CXCL2/MIP-2 and Neutralization by Mouse CXCR2/IL-8 RB Antibody. Recombinant Mouse CXCL2/MIP-2 (Catalog # 452-M2) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB2164). The ND50 is typically 15-50 µg/mL.