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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Flow cytometry [2]
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- Product number
- FAB2164A-025 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CXCR2/IL-8RB APC-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Stains mouse CXCR2-transfected cells but not CXCR1 transfectants.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Red dye
- Antigen sequence
P35343- Isotype
- IgG
- Antibody clone number
- 242216
- Vial size
- 25 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Rac GTPase Activating Protein ARHGAP25 Regulates Leukocyte Transendothelial Migration in Mice.
CXCR1/2 inhibition blocks and reverses type 1 diabetes in mice.
Staphylococcus aureus Staphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis.
Csépányi-Kömi R, Wisniewski É, Bartos B, Lévai P, Németh T, Balázs B, Kurz AR, Bierschenk S, Sperandio M, Ligeti E
Journal of immunology (Baltimore, Md. : 1950) 2016 Oct 1;197(7):2807-15
Journal of immunology (Baltimore, Md. : 1950) 2016 Oct 1;197(7):2807-15
CXCR1/2 inhibition blocks and reverses type 1 diabetes in mice.
Citro A, Valle A, Cantarelli E, Mercalli A, Pellegrini S, Liberati D, Daffonchio L, Kastsiuchenka O, Ruffini PA, Battaglia M, Allegretti M, Piemonti L
Diabetes 2015 Apr;64(4):1329-40
Diabetes 2015 Apr;64(4):1329-40
Staphylococcus aureus Staphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis.
Laarman AJ, Mijnheer G, Mootz JM, van Rooijen WJ, Ruyken M, Malone CL, Heezius EC, Ward R, Milligan G, van Strijp JA, de Haas CJ, Horswill AR, van Kessel KP, Rooijakkers SH
The EMBO journal 2012 Aug 29;31(17):3607-19
The EMBO journal 2012 Aug 29;31(17):3607-19
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CXCR2/IL-8 RB in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse Gr-1/Ly-6G Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1037F) and either (A) Rat Anti-Mouse CXCR2/IL-8 RB APC-conjugated Monoclonal Antibody (Catalog # FAB2164A) or (B) Rat IgG1 Allophycocyanin Isotype Control (Catalog # IC005A). View our protocol for Staining Membrane-associated Proteins.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CXCR2/IL-8 RB in HEK293 Human Cell Line Transfected with Mouse CXCR2/IL-8 RB and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse CXCR2/IL-8 RB or (B) mouse CXCR1/IL-8 RA and eGFP was stained with Rat Anti-Mouse CXCR2/IL-8 RB APC-conjugated Monoclonal Antibody (Catalog # FAB2164A). Quadrant markers were set based on control antibody staining (Catalog # IC005A). View our protocol for Staining Membrane-associated Proteins.
