14-1829-82

antibody from Invitrogen Antibodies

Targeting: CXCR2 CD182, CMKAR2, IL8RB

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14-1829-82

Provider

Invitrogen Antibodies

Product name

CD182 (CXCR2) Monoclonal Antibody (eBio5E8-C7-F10 (5E8-C7-F10)), eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The eBio5E8-C7-F10 monoclonal antibody reacts with human CD182 (CXCR2, IL-8Rbeta). CD182 is a 67-70 kDa member of the seven-transmembrane spanning G-protein coupled receptor (GPCR) family. CD182 is expressed as a homodimer, or a heterodimer with CD181 (CXCR1, IL-8Ralpha) and is expressed on granulocytes, NK cells, a subset of T cells, mast cells, monocytes, endothelial cells, megakaryocytes and oligodendrocytes. Binding of it's ligands, which include IL-8, NAP-2, GCP-2 and GRO-alpha, -beta and -gamma, induces several biological outcomes such as cell activation, chemotaxis, proliferation and angiogenesis. There are several functional differences between CD181 and CD182. Both receptors are able to mediate chemotaxis and intracellular calcium changes, but only CD181 mediates phospolipase D activation and respiratory burst. Furthermore, studies have shown that IL-8 predominantly mediates its effects on neutrophil function through CD181. Applications Reported: This eBio5E8-C7-F10 (5E8-C7-F10) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio5E8-C7-F10 (5E8-C7-F10) antibody has been tested by flow cytometric analysis of normal human peripheral blood. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

eBio5E8-C7-F10 (5E8-C7-F10)

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

4° C

References

Submitted references

n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils.

Wu B, Wang L, Jiang L, Dong L, Xu F, Lu Y, Jin J, Wang Z, Liang G, Shan X
Molecular medicine reports 2018 Jan;17(1):179-185

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Type I IFNs induce anti-tumor polarization of tumor associated neutrophils in mice and human.

Andzinski L, Kasnitz N, Stahnke S, Wu CF, Gereke M, von Köckritz-Blickwede M, Schilling B, Brandau S, Weiss S, Jablonska J
International journal of cancer 2016 Apr 15;138(8):1982-93

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Anomalous type 17 response to viral infection by CD8+ T cells lacking T-bet and eomesodermin.

Intlekofer AM, Banerjee A, Takemoto N, Gordon SM, Dejong CS, Shin H, Hunter CA, Wherry EJ, Lindsten T, Reiner SL
Science (New York, N.Y.) 2008 Jul 18;321(5887):408-11

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Staining of normal human peripheral blood cells with 0.25 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (left) or 0.25 µg of Anti-Human CD182 (CXCR2) Purified (right) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4012). Cells in the large scatter population were used for analysis.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL