Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- MA5-17711 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD35 Monoclonal Antibody (To5)
- Antibody type
- Monoclonal
- Description
- MA5-17711 targets CD35/CR1 in FACS, IHC (F), and WB applications and shows reactivity with Human samples.
- Antibody clone number
- To5
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraformaldehyde-fixed paraffin-embedded human spleen (A) and human tonsil (B) sections at 400x magnification with DAPI counterstain. Primary antibody used was a CD35/CR1 monoclonal antibody (clone To5) at a 1:500 dilution, followed by secondary antibody was a 488 fluorescent conjugated Goat anti-mouse Ig at a 1:500 dilution and Normal Goat Serum (blocking)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of human peripheral blood lymphocytes were stained using a CD35/CR1 monoclonal antibody (Product # MA5-17711) (filled histogram) or mouse IgG1 isotype control (open histogram).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Human Peripheral Blood Leukocytes stained using anti-CD35/CR1 Monoclonal Antibody (Product # MA5-17711) at a 0.5 µg/10^6 cells dilution. Primary antibody was visualization with a secondary antibody - Goat Anti-Mouse FITC at a 1:500 dilution.