Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 17-0359-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD35 Monoclonal Antibody (E11), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This E11 monoclonal antibody reacts with human and non-human primate CD35, which is also known as complement receptor 1 (CR1). CD35 is expressed on B cells, T cell subsets, follicular dendritic cells, macrophages, neutrophils, eosinophils, and erythrocytes. This cell surface protein is also present on non-immune cells such as glomerular podocytes and Langerhans cells. CD35 binds the complement components C3b and C4b, which are derived from cleavage of C3 and C4, respectively, in the classical and alternative complement pathways. In humans, CD35 has been implicated in a variety of roles, including complement activation, transporting C3 complexes on erythrocytes, phagocytosis of complement-bound neutrophils and macrophages, B cell proliferation, and opsonization. Reports also indicate that Plasmodium falciparum, the pathogen that causes malaria, invades host erythrocytes by binding CD35. Decreased expression of CD35 on erythrocytes has been associated with systemic lupus erythematosus (SLE), type I diabetes, and rheumatoid arthritis. The E11 antibody has been reported to crossreact to capuchin monkey, chimpanzee, cynomologus monkey, olive baboon, and rhesus macaque. Applications Reported: This E11 antibody has been reported for use in flow cytometric analysis. Applications Tested: This E11 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- E11
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references A group B Streptococcus alpha-like protein subunit vaccine induces functionally active antibodies in humans targeting homotypic and heterotypic strains.
Complement receptor 1 is the host erythrocyte receptor for Plasmodium falciparum PfRh4 invasion ligand.
Ligation of complement receptor 1 increases erythrocyte membrane deformability.
Complement Receptor 1: disease associations and therapeutic implications.
Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1).
Pawlowski A, LannergÄrd J, Gonzalez-Miro M, Cao D, Larsson S, Persson JJ, Kitson G, Darsley M, Rom AL, Hedegaard M, Fischer PB, Johansson-Lindbom B
Cell reports. Medicine 2022 Feb 15;3(2):100511
Cell reports. Medicine 2022 Feb 15;3(2):100511
Complement receptor 1 is the host erythrocyte receptor for Plasmodium falciparum PfRh4 invasion ligand.
Tham WH, Wilson DW, Lopaticki S, Schmidt CQ, Tetteh-Quarcoo PB, Barlow PN, Richard D, Corbin JE, Beeson JG, Cowman AF
Proceedings of the National Academy of Sciences of the United States of America 2010 Oct 5;107(40):17327-32
Proceedings of the National Academy of Sciences of the United States of America 2010 Oct 5;107(40):17327-32
Ligation of complement receptor 1 increases erythrocyte membrane deformability.
Glodek AM, Mirchev R, Golan DE, Khoory JA, Burns JM, Shevkoplyas SS, Nicholson-Weller A, Ghiran IC
Blood 2010 Dec 23;116(26):6063-71
Blood 2010 Dec 23;116(26):6063-71
Complement Receptor 1: disease associations and therapeutic implications.
Khera R, Das N
Molecular immunology 2009 Feb;46(5):761-72
Molecular immunology 2009 Feb;46(5):761-72
Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1).
Hogg N, Ross GD, Jones DB, Slusarenko M, Walport MJ, Lachmann PJ
European journal of immunology 1984 Mar;14(3):236-43
European journal of immunology 1984 Mar;14(3):236-43
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD19 eFluor® 450 (Product # 48-0199-42) and Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human/Non-Human Primate CD35 APC (right). Cells in the lymphocyte gate were used for analysis.