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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- 700582 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-PKC lambda/iota (Thr555, Thr563) Recombinant Rabbit Monoclonal Antibody (31H34L29)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 31H34L29
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-PKC iota pThr555/PKC lambda pThr563 in HEK293 cell lysate (30 µg/lane) using a Phospho-PKC iota pThr555/PKC lambda pThr563 recombinant rabbit monoclonal antibody (Product # 700582) at a dilution of 5 µg/mL. NBT/BCIP was used as the substrate (Product # WB7105).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of PRKC1 (PT555)/ (PT563) showing staining in the cytoplasm of paraffin-embedded mouse skeletal muscle (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a PRKC1 (PT555)/ (PT563) Recombinant Rabbit Monoclonal Antibody (Product # 700582) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
