PA5-65216

antibody from Invitrogen Antibodies

Targeting: PRKCI DXS1179E, PKCI

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-65216

Provider

Invitrogen Antibodies

Product name

PKC lambda/iota Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: STMSHTVAGGG SGDHSHQVRV KAYYRGDIMI THFEPSISFE GLCNEVRDMC SFDNEQLFTM KWIDEEGDPC TVSSQLEL

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of PKC lambda/iota was achieved by transfecting C2C12 with PKC lambda/iota specific siRNAs (Silencer® select Product # s71706 and s71707). Western blot analysis (Fig. a) was performed using nuclear enriched extracts from the PKC lambda/iota knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with PKC lambda/iota Polyclonal Antibody (Product # PA5-65216, 1 µg/mL) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to PKC lambda/iota. An uncharacterized band (*) was observed at around 48 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of PKC lambda/iota in human cell line U-2 OS shows localization to cytosol, microtubules and cytokinetic bridge. Samples were probed using a PKC lambda/iota Polyclonal Antibody (Product # PA5-65216).