CABT-B9297

antibody from Creative Diagnostics

Targeting: PRKCI DXS1179E, PKCI

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

CABT-B9297

Provider

Creative Diagnostics

Product name

Mouse anti-Human PKCÖñ monoclonal antibody, clone 52/QLDÖñ

Antibody type

Monoclonal

Description

The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. These proteins are products of multiple genes and alternative splicing. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with the PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester. However, the novel PKC (nPKC) subfamily members ( Ñ´, š÷, »Ò, and IJ isoforms) and the atypical PKC (aPKC) subfamily members (šø, ?, and Öñ isoforms) are Ca2+ independent and lack the C2 domain. The aPKC members are unique in that their activity is independent of diacylglycerols and phorbol esters. They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC. The C-terminal region of PKC contains the catalytic domain. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters, and growth factors. PKCÖñ shows the highest degree of amino acid homology with PKCšø (72%) and PKCÖñ mRNA is expressed in a variety of cells and tissues. The PKCÖñ protein kinase is capable of autophosphorylation and can be activated by phosphatidylserine, but not by other PKC activators such as diacylglycerols, Ca2+, or phorbol esters.

Reactivity

Human, Mouse, Rat, Canine, Chicken/Avian

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

52/QLDÖñ

Vial size

50ug, 150ug

Concentration

250 Ã×g/ml

Storage

Store undiluted at -20°C.