Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- NB600-1397 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-1397, RRID:AB_2287663
- Product name
- Mouse Monoclonal Transportin 1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Bovine, Canine, Rabbit, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references A defect in the RNA-processing protein HNRPDL causes limb-girdle muscular dystrophy 1G (LGMD1G).
Regulation of heterogenous nuclear ribonucleoprotein A1 transport by phosphorylation in cells stressed by osmotic shock.
Two separate regions essential for nuclear import of the hnRNP D nucleocytoplasmic shuttling sequence.
Identification of the nucleocytoplasmic shuttling sequence of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its interaction with mRNA.
Transportin-mediated nuclear import of heterogeneous nuclear RNP proteins.
Transportin-mediated nuclear import of heterogeneous nuclear RNP proteins.
Vieira NM, Naslavsky MS, Licinio L, Kok F, Schlesinger D, Vainzof M, Sanchez N, Kitajima JP, Gal L, Cavaçana N, Serafini PR, Chuartzman S, Vasquez C, Mimbacas A, Nigro V, Pavanello RC, Schuldiner M, Kunkel LM, Zatz M
Human molecular genetics 2014 Aug 1;23(15):4103-10
Human molecular genetics 2014 Aug 1;23(15):4103-10
Regulation of heterogenous nuclear ribonucleoprotein A1 transport by phosphorylation in cells stressed by osmotic shock.
Allemand E, Guil S, Myers M, Moscat J, Cáceres JF, Krainer AR
Proceedings of the National Academy of Sciences of the United States of America 2005 Mar 8;102(10):3605-10
Proceedings of the National Academy of Sciences of the United States of America 2005 Mar 8;102(10):3605-10
Two separate regions essential for nuclear import of the hnRNP D nucleocytoplasmic shuttling sequence.
Suzuki M, Iijima M, Nishimura A, Tomozoe Y, Kamei D, Yamada M
The FEBS journal 2005 Aug;272(15):3975-87
The FEBS journal 2005 Aug;272(15):3975-87
Identification of the nucleocytoplasmic shuttling sequence of heterogeneous nuclear ribonucleoprotein D-like protein JKTBP and its interaction with mRNA.
Kawamura H, Tomozoe Y, Akagi T, Kamei D, Ochiai M, Yamada M
The Journal of biological chemistry 2002 Jan 25;277(4):2732-9
The Journal of biological chemistry 2002 Jan 25;277(4):2732-9
Transportin-mediated nuclear import of heterogeneous nuclear RNP proteins.
Siomi MC, Eder PS, Kataoka N, Wan L, Liu Q, Dreyfuss G
The Journal of cell biology 1997 Sep 22;138(6):1181-92
The Journal of cell biology 1997 Sep 22;138(6):1181-92
Transportin-mediated nuclear import of heterogeneous nuclear RNP proteins.
Siomi MC, Eder PS, Kataoka N, Wan L, Liu Q, Dreyfuss G
The Journal of cell biology 1997 Sep 22;138(6):1181-92
The Journal of cell biology 1997 Sep 22;138(6):1181-92
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Transportin 1 Antibody (D45) [NB600-1397] - Whole cell protein from A431, Hek293 and MCF7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Transportin 1 (D45) in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Transportin 1 Antibody (D45) [NB600-1397] - Human lung cancer cell line usig lysates from HCC44 cell line. (N: Nuclear fraction, C: Cytoplasmic fraction). Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Transportin 1 Antibody (D45) [NB600-1397] - IHC analysis of a formalin fixed paraffin embedded tissue section of human pancreas using Transportin 1 antibody (clone D45) at 1:200 dilution. The antibody generated specific nuclear and cytoplasmic signal of Transportin 1 protein in the glandular cells while the connective tissue was largely negative.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Transportin 1 Antibody (D45) [NB600-1397] - IHC analysis of a formalin fixed paraffin embedded tissue section of human pancreas using Transportin 1 antibody (clone D45) at 1:200 dilution. The antibody generated specific nuclear and cytoplasmic signal of Transportin 1 protein in the glandular cells while the connective tissue was largely negative.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): Transportin 1 Antibody (D45) [NB600-1397] - HeLa cells were stained with Transportin 1 NB600-1397 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody.