Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NBP2-14868 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Dystroglycan Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Chronic Graft Versus Host Myopathies: Noninflammatory, Multi-Tissue Pathology With Glycosylation Disorders.
Detection of variants in dystroglycanopathy-associated genes through the application of targeted whole-exome sequencing analysis to a large cohort of patients with unexplained limb-girdle muscle weakness.
Pestronk A
Journal of neuropathology and experimental neurology 2020 Jan 1;79(1):102-112
Journal of neuropathology and experimental neurology 2020 Jan 1;79(1):102-112
Detection of variants in dystroglycanopathy-associated genes through the application of targeted whole-exome sequencing analysis to a large cohort of patients with unexplained limb-girdle muscle weakness.
Johnson K, Bertoli M, Phillips L, Töpf A, Van den Bergh P, Vissing J, Witting N, Nafissi S, Jamal-Omidi S, Łusakowska A, Kostera-Pruszczyk A, Potulska-Chromik A, Deconinck N, Wallgren-Pettersson C, Strang-Karlsson S, Colomer J, Claeys KG, De Ridder W, Baets J, von der Hagen M, Fernández-Torrón R, Zulaica Ijurco M, Espinal Valencia JB, Hahn A, Durmus H, Willis T, Xu L, Valkanas E, Mullen TE, Lek M, MacArthur DG, Straub V
Skeletal muscle 2018 Jul 30;8(1):23
Skeletal muscle 2018 Jul 30;8(1):23
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Dystroglycan Antibody [NBP2-14868] - U87-MG whole cell extracts and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DAG1 antibody diluted at 1:500.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Dystroglycan Antibody [NBP2-14868] - Various tissue extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dystroglycan Antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Dystroglycan Antibody [NBP2-14868] - MCF-7 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dystroglycan Antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Alpha Dystroglycan Antibody [NBP2-14868] - Sample: Paraffin-embedded colon carcinoma.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Dystroglycan Antibody [NBP2-14868] - Mouse placenta. DAG1 stained by Dystroglycan antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.