GTX105038

antibody from GeneTex

Targeting: DAG1 156DAG, A3a, AGRNR, DAG

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX105038

Provider

GeneTex

Proper citation

GeneTex Cat#GTX105038, RRID:AB_2036733

Product name

DAG1 antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Horse

Host

Rabbit

References

Submitted references

Absence of α- and β-dystroglycan is associated with Walker-Warburg syndrome.

Riemersma M, Mandel H, van Beusekom E, Gazzoli I, Roscioli T, Eran A, Gershoni-Baruch R, Gershoni M, Pietrokovski S, Vissers LE, Lefeber DJ, Willemsen MA, Wevers RA, van Bokhoven H
Neurology 2015 May 26;84(21):2177-82

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Disease mutations in CMP-sialic acid transporter SLC35A1 result in abnormal α-dystroglycan O-mannosylation, independent from sialic acid.

Riemersma M, Sandrock J, Boltje TJ, Büll C, Heise T, Ashikov A, Adema GJ, van Bokhoven H, Lefeber DJ
Human molecular genetics 2015 Apr 15;24(8):2241-6

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Transcriptome profiling of granulosa and theca cells during dominant follicle development in the horse.

Donadeu FX, Fahiminiya S, Esteves CL, Nadaf J, Miedzinska K, McNeilly AS, Waddington D, Gérard N
Biology of reproduction 2014 Nov;91(5):111

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Virus entry. Lassa virus entry requires a trigger-induced receptor switch.

Jae LT, Raaben M, Herbert AS, Kuehne AI, Wirchnianski AS, Soh TK, Stubbs SH, Janssen H, Damme M, Saftig P, Whelan SP, Dye JM, Brummelkamp TR
Science (New York, N.Y.) 2014 Jun 27;344(6191):1506-10

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: U87-MG 7.5% SDS PAGE GTX105038 diluted at 1:500

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

DAG1 antibody detects DAG1 protein by western blot analysis. U87-MG whole cell extracts and membrane extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DAG1 antibody (GTX105038) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed A431, using alpha Dystroglycan(GTX105038) antibody at 1:500 dilution.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

alpha Dystroglycan antibody detects alpha Dystroglycan protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: alpha Dystroglycan protein stained by alpha Dystroglycan antibody (GTX105038) diluted at 1:1000.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

alpha Dystroglycan antibody detects DAG1 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. alpha Dystroglycan antibody (GTX105038) dilution: 1:500.