A010-200

antibody from Badrilla Ltd

Targeting: DAG1 156DAG, A3a, AGRNR, DAG

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

A010-200

Provider

Badrilla Ltd

Product name

Anti-beta dystroglycan

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

3kDa Dystrophin-associated glycoprotein; (43-DAG, DG, b-DAG, b -DG, DAG1) β-dystroglycan is a component of the dystrophin glycoprotein complex (DGC), a membrane-spanning complex that links the interior cytoskeleton to the extracellular matrix in muscle and other tissues (Winder 2001). It is a transmembrane protein (single TM domain) of 34.9kDa molecular weight, and pI 8.85, which displays an apparent molecular weight of 43kDa by SDS-PAGE due to asparagine-linked glycosylation and a high proportion of proline residues in the cytoplasmic domain. The dystroglycan complex is a subcomplex within the DGC comprising alpha and beta-subunits. Dystroglycan is of importance in cell signalling, cell polarity, cell adhesion and movement. β-dystroglycan binds to dystrophin (in muscle cells) and utrophin (in non-muscle cells), but dissociates from these proteins when phosphorylated on Tyr-892 allowing its association with SH2 containing proteins such as Src and other cytolinker proteins such as plectin. This antibody recognises Tyr-892 phosphorylated forms of β-dystroglycan (Tyr-892 in human, Tyr-890 in mouse)

Host

Rabbit

Antigen sequence

MTPYRSPPPY(PO3H2)VP


Isotype

IgG

Vial size

100 µl

Storage

Lyophilised: 4 °C Reconstituted:-80 °C

Handling

Reconstitute lyophilised powder in 100 µl of 18 MΩ H2O, aliquot and store frozen at -80 °C for 1 year. Avoid freeze - thaw cycles