11017-1-AP

antibody from Invitrogen Antibodies

Targeting: DAG1 156DAG, A3a, AGRNR, DAG

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11017-1-AP

Provider

Invitrogen Antibodies

Product name

Dystroglycan Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: VGEKSWVQF NSNSQLMYGL PDSSHVGKHE YFMHATDKGG LSAVDAFEIH VHRRPQGDRA PARFKAKFVG DPALVLNDIH KKIALVKKLA FAFGDRNCST ITLQNITRGS IVVEWTNNTL PLEPCPKEQI AGLSRRIAED DGKPRPAFSN ALEPDFKATS ITVTGSGSCR HLQFIPVVPP RRVPSEAPPT EVPDRDPEKS SEDDVYLHTV IPAVVVAAIL LIAGIIAMIC YRKKRKGKLT LEDQATFIKK GVPIIFADEL DDSKPPPSSS MPLILQEEKA PLPPPEYPNQ SVPETTPLNQ DTMGEYTPLR DEDPNAPPYQ PPPPFTAPME GKGSRPKNMT PYRSPPPYVP P (546-895 aa encoded by BC012740)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 11017-1-AP ( Dystroglycan antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 11017-1-AP (Dystroglycan antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11017-1-AP (Dystroglycan antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 11017-1-AP (Dystroglycan antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 11017-1-AP (Dystroglycan antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse heart tissue slide using 11017-1-AP (Dystroglycan antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse heart tissue slide using 11017-1-AP (Dystroglycan antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse heart tissue slide using 11017-1-AP (Dystroglycan antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse skeletal muscle tissue slide using 11017-1-AP (Dystroglycan antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse skeletal muscle tissue slide using 11017-1-AP (Dystroglycan antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer using 11017-1-AP (Dystroglycan antibody) at dilution of 1:10 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-Dystroglycan (IP:11017-1-AP, 4ug; Detection:11017-1-AP 1:500) with mouse brain tissue lysate 2640ug.