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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [5]
- Immunohistochemistry [2]
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- Product number
- PA5-28179 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- alpha Dystroglycan Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: U87-MG, U87-MG membrane fraction extract. Predicted reactivity: Mouse (92%), Rat (92%), Dog (92%), Cat (95%), Rabbit (94%), Rhesus Monkey (98%), Bovine (93%).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 1.28 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of alpha Dystroglycan in paraformaldehyde-fixed A431 cells using an Alpha Dystroglycan polyclonal antibody (Product # PA5-28179) at a 1:500 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of alpha Dystroglycan was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Dystroglycan Polyclonal Antibody (Product # PA5 28179) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- alpha Dystroglycan Polyclonal Antibody detects DAG1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DAG1 stained by alpha Dystroglycan Polyclonal Antibody (Product # PA5-28179) diluted at 1:500. Red: Tau, stained by Phospho-Tau (Ser262) Polyclonal Antibody [GT287]diluted at 1:500. Blue: Fluoroshield with DAPI .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- alpha Dystroglycan Polyclonal Antibody detects DAG1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DAG1 stained by alpha Dystroglycan Polyclonal Antibody (Product # PA5-28179) diluted at 1:500. Red: Tau, stained by Phospho-Tau (Ser262) Polyclonal Antibody [GT287]diluted at 1:500. Blue: Fluoroshield with DAPI .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of alpha Dystroglycan was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Dystroglycan Polyclonal Antibody (Product # PA5 28179) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- alpha Dystroglycan Polyclonal Antibody detects DAG1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse placenta. DAG1 stained by alpha Dystroglycan Polyclonal Antibody (Product # PA5-28179) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of alpha Dystroglycan was performed in paraffin-embedded mouse placenta tissue using alpha Dystroglycan Polyclonal Antibody (Product # PA5-28179) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
