- PA5-28179 - Invitrogen Antibodies DAG1 antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of alpha Dystroglycan using 30 µg of U87-MG lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an Alpha Dystroglycan polyclonal antibody (Product # PA5-28179) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of DAG1 in U87-MG whole cell extracts and membrane extracts (30 µg). Samples were separated by 7.5% SDS-PAGE and the membrane was probed with DAG1 Polyclonal antibody (Product # PA5-28179) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western Blot analysis of alpha Dystroglycan was performed by separating 30 µg of Various tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a alpha Dystroglycan Polyclonal Antibody (Product # PA5-28179) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western Blot analysis of alpha Dystroglycan was performed by separating 30 µg of MCF-7 whole cell and membrane extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a alpha Dystroglycan Polyclonal Antibody (Product # PA5-28179) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using alpha Dystroglycan Polyclonal Antibody (Product # PA5-28179). Human tissue extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Dystroglycan Polyclonal Antibody (Product # PA5-28179) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: alpha Dystroglycan Polyclonal Antibody detects DAG1 protein by western blot analysis. U87-MG whole cell extracts and membrane extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Dystroglycan Polyclonal Antibody (Product # PA5-28179) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using alpha Dystroglycan Polyclonal Antibody (Product # PA5-28179). Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Dystroglycan Polyclonal Antibody (Product # PA5-28179) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot was performed using Anti-alpha Dystroglycan Polyclonal Antibody (Product # PA5-28179) and a band around 200kDa corresponding to alpha Dystroglycan was observed across cell lines tested except MDA-MB-231 which is reported to be low for Dystroglycan A expression. An additional uncharacterized band (*) was observed across the cell lines. Membrane enriched extracts (60 µg lysate) of MCF7 (Lane 1), SK-BR-3 (Lane 2) and MDA-MB-231 (Lane 3) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescent analysis of alpha Dystroglycan in paraformaldehyde-fixed A431 cells using an Alpha Dystroglycan polyclonal antibody (Product # PA5-28179) at a 1:500 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunocytochemistry-Immunofluorescence analysis of alpha Dystroglycan was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha Dystroglycan Polyclonal Antibody (Product # PA5 28179) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: alpha Dystroglycan Polyclonal Antibody detects DAG1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DAG1 stained by alpha Dystroglycan Polyclonal Antibody (Product # PA5-28179) diluted at 1:500. Red: Tau, stained by Phospho-Tau (Ser262) Polyclonal Antibody [GT287]diluted at 1:500. Blue: Fluoroshield with DAPI .

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemistry (Paraffin) analysis of alpha Dystroglycan was performed in paraffin-embedded mouse placenta tissue using alpha Dystroglycan Polyclonal Antibody (Product # PA5-28179) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: alpha Dystroglycan Polyclonal Antibody detects DAG1 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse placenta. DAG1 stained by alpha Dystroglycan Polyclonal Antibody (Product # PA5-28179) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

PA5-28179