674902

ACAN antibody from BioLegend
AGC1, CSPG1, CSPGCP, MSK16

Antibody data

Product number
674902
Provider
BioLegend
Proper citation
BioLegend Cat#674902, RRID:AB_2565486
Product name
Purified anti-Aggrecan
Provider product page
BioLegend - 674902
Antibody type
Monoclonal
Antigen
Recombinant human Aggrecan produced in 293E cells.
Description
Aggrecan, also known as aggrecan 1, is the major proteoglycan in the articular cartilage. This high molecular weight protein exhibits a bottlebrush structure, in which chondroitin sulfate and keratan sulfate chains are attached to an extended protein core. Aggrecan plays an important role in the proper functioning of articular cartilage by providing a hydrated gel structure through its interaction with hyaluronan and link proteins. Aggrecan is expressed by chondrocytes and is found to be crucial in chondroskeletal morphogenesis during development. Mutations in this gene may be involved in skeletal dysplasia and spinal cord degeneration. Structurally, aggrecan is composed of two globular domains (G1 and G2) at the N-terminal end and one globular domain (G3) at the C-terminal end, which are separated by a large extended domain (CS) heavily modified with glycosaminoglycans. G1 and G2 domains of aggrecan are separated by the interglobular domain (IGD). Biologically, the G1 domain interacts with hyaluronan acid and link proteins, which forms stable ternary complexes in the extracellular matrix (ECM). The G2 domain is homologous to the tandem repeats of G1 and link proteins, and is involved in product processing. G3 makes up the carboxyl terminus of the core protein, and enhances glycosaminoglycan modification and product secretion. Also, the G3 domain links the proteoglycan aggregates to the ECM proteins, which are fibulins and tenascins. The degradation of aggrecan appears to initiate at the C-terminus. The population of aggrecan molecules without the G3 domain increases with aging.
Reactivity
Human
Host
Mouse
Conjugate
Unconjugated
Antibody clone number
M4004A03
Vial size
100 µg
Concentration
0.5 mg/ml
Storage
2°C-8°C
Handling
Ambient RT
Provider Type Product Number
- No reagents -