AHP0022

antibody from Invitrogen Antibodies

Targeting: ACAN AGC1, CSPG1, CSPGCP, MSK16

Provider product page for AHP0022

Western blot

ELISA

Immunohistochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [35]
Comments [0]
Validations
Other assay [9]

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Product number: AHP0022 - Provider product page
Provider: Invitrogen Antibodies
Product name: Aggrecan Monoclonal Antibody (969D4D11)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: This product is recommended as a detection antibody in Sandwich ELISA applications. This clone can also be referred to as clone number 969D 4D11 2A9.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 969D4D11
Vial size: 500 µL
Concentration: 1 mg/mL
Storage: 4° C

ACAN protein structure - AHP0022 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Specificity and technical performance of the aggrecan capture OA-1 ARGS ELCL assay . (a) 2.4 mug of ADAMTS-4-digested and deglycosylated human aggrecan was separated on a 3 to 8% Tris-acetate gel, transferred and probed with anti-G1, anti-aggrecan (AHP0022), or anti-ARGS (OA-1). Protein standard molecular weights and aggrecan fragments detected are indicated. Epitopes recognized by the antibodies are written in color: red, anti-G1; blue, anti-aggrecan (AHP0022); green, anti-ARGS. Aggrecan domains and amino acid sequences: G1 and G2, globular domains 1 and 2; CS1 and CS2, chondroitin sulfate-rich domains 1 and 2; TEGE and ARGS: C- and N-terminal amino acid sequences at the aggrecanase cleavage site within the interglobular domain; SELE, C-terminal amino acid sequence of aggrecanase cleavage within the CS2 [ 1 ]. (b) ARGS concentration measured with the OA-1 ARGS ELCL assay and the BC-3 ARGS ELISA [ 35 ] in 43 individual SFs with a regression line, dashed line of equality, and Pearson's correlation coefficient ( r ). (c) Dilution curves of ARGS-aggrecan standard and four synovial fluids (SFs) analyzed in the OA-1 ARGS ELCL assay. Sample concentrations of ARGS-aggrecan at different dilutions were calculated from the standard curve (four-parameter logistic). Dilutions falling within the range of detection of the standard curve (solid symbols within horizontal lines) were used for linearity of dilution calculations (Table 2), with mean values presented in the legend.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 1 Immunophenotypic and morphological features of MSCs and chondrocytes in direct co-culture. (A) Immunohistochemical staining of type I, II, X collagen and aggrecan in MSCs/chondrocytes after 17 days of differentiation in direct 2D and 3D co-culture model (EnVision technique, scale bar = 50 um); (B) Chondrocytes and (G) direct co-culture on culture flask (inverted phase contrast microscope, scale bar = 100 um); (C) Hematoxylin-eosin staining of chondroinduced MSCs and (D) co-culture in 2D culture after 14 days (scale bar = 50 um); (E) Expression of vimentin in MSCs/chondrocytes and (F) chondroinduced MSCs after 17 days of culture in direct 2D co-culture model (EnVision technique, scale bar = 50 um); (H) The viability of MSCs and chondrocytes on the Hyaff-11 membrane after 48 h. Data are given as mean +- standard deviation.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 2 Immunohistochemical staining of type I, II collagen and aggrecan in MSCs in standard culture and MSCs/chondrocytes in direct co-culture model after 17 days differentiation on Hyaff-11 membrane (EnVision technique, scale bar = 50 um, co-culture collagen I scale bar = 100 um).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 8. Composition of tissue bridge formed by migratory cells. Immunohistochemical staining of tissue sections using antibodies reactive with (A) COL2, (B) ACAN, (C) COL1, or (D) MMP-13. (E) Isotype negative controls for COL2, ACAN, COL1, and MMP-13. *Bioengineered cartilage. #Native cartilage. The white box indicates the site of the magnified image of the tissue bridge. N = 3 biological replicates, (1 technical replicate). ACAN, aggrecan; COL, collagen; MMP, matrix metalloprotease.