Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [29]
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- Product number
- LS-C483023 - Provider product page
- Provider
- LSBio
- Product name
- ACAN / Aggrecan Antibody (aa200-250) LS-C483023
- Antibody type
- Polyclonal
- Description
- Antiserum
- Reactivity
- Human
- Host
- Rabbit
- Storage
- Maintain lyophilized and reconstituted antibodies at -20°C for long term storage and at 2°C to 8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze/thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse cerebellum tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse cerebellum tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse cerebellum tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse cerebellum tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse cerebellum tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse cerebellum tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse cerebellum tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer's instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse cerebellum tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse cerebellum tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse cerebellum tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse cerebellum tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse cerebellum tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse cerebellum tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Rabbit antibody to Aggrecan (200-250). IHC on paraffin sections of mouse spinal cord tissue using Rabbit antibody to Aggrecan (200-250). HIER: 1 mM EDTA, pH 8 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturer\'s instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT (using Autostainer) Sections were counterstained with Harris Hematoxylin.