Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF5430 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal Complement Factor D/Adipsin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Complement Factor D/Adipsin in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) Complement Factor D is observed and less than 5% cross-reactivity with rhGranzyme A is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Cell-Type-Specific Complement Expression in the Healthy and Diseased Retina.
Contribution of Adipose-Derived Factor D/Adipsin to Complement Alternative Pathway Activation: Lessons from Lipodystrophy.
Regulation of C3 Activation by the Alternative Complement Pathway in the Mouse Retina.
Complement factor B is the downstream effector of TLRs and plays an important role in a mouse model of severe sepsis.
Pauly D, Agarwal D, Dana N, Schäfer N, Biber J, Wunderlich KA, Jabri Y, Straub T, Zhang NR, Gautam AK, Weber BHF, Hauck SM, Kim M, Curcio CA, Stambolian D, Li M, Grosche A
Cell reports 2019 Nov 26;29(9):2835-2848.e4
Cell reports 2019 Nov 26;29(9):2835-2848.e4
Contribution of Adipose-Derived Factor D/Adipsin to Complement Alternative Pathway Activation: Lessons from Lipodystrophy.
Wu X, Hutson I, Akk AM, Mascharak S, Pham CTN, Hourcade DE, Brown R, Atkinson JP, Harris CA
Journal of immunology (Baltimore, Md. : 1950) 2018 Apr 15;200(8):2786-2797
Journal of immunology (Baltimore, Md. : 1950) 2018 Apr 15;200(8):2786-2797
Regulation of C3 Activation by the Alternative Complement Pathway in the Mouse Retina.
Williams JA, Stampoulis D, Gunter CE, Greenwood J, Adamson P, Moss SE
PloS one 2016;11(8):e0161898
PloS one 2016;11(8):e0161898
Complement factor B is the downstream effector of TLRs and plays an important role in a mouse model of severe sepsis.
Zou L, Feng Y, Li Y, Zhang M, Chen C, Cai J, Gong Y, Wang L, Thurman JM, Wu X, Atkinson JP, Chao W
Journal of immunology (Baltimore, Md. : 1950) 2013 Dec 1;191(11):5625-35
Journal of immunology (Baltimore, Md. : 1950) 2013 Dec 1;191(11):5625-35
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse Complement Factor D/Adipsin by Western Blot. Western blot shows lysates of mouse plasma. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Complement Factor D/Adipsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5430) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Complement Factor D/Adipsin at approximately 45kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.