600-401-411

antibody from Rockland Immunochemicals, Inc.

Targeting: PDK1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-411

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-411, RRID:AB_217935

Product name

Anti-PDK1 (RABBIT) Antibody - 600-401-411

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's affinity purified anti-PDK-1 antibody shows detection of myc-tagged human PDK-1 at 60kDa in ~10 µg of a virus infected Sf9 cell lysate (arrow). The nitrocellulose membrane was probed overnight at 4° C with the primary antibody diluted 1:750 in 1% non-fat dry milk. HRP conjugated Goat-anti-Rabbit IgG [H&L] (p/n 611-1302) and chemiluminescent detection is recommended. Other detection systems will yield similar results.

Validation comment

Western Blot