MAB3576

antibody from Novus Biologicals

Targeting: DUSP6 MKP-3, PYST1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB3576

Provider

Novus Biologicals

Product name

Mouse Monoclonal MKP-3/DUSP6 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects endogenous human, mouse, and rat MKP-3 in Western blots. In Western blots, this antibody does not cross-react with recombinant human MKP-1 or MKP-2.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Inhibition of mitogen-activated protein kinase phosphatase 3 activity by interdomain binding.

Mark JK, Aubin RA, Smith S, Hefford MA
The Journal of biological chemistry 2008 Oct 17;283(42):28574-83

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human and Mouse MKP-3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and CTLL-2 mouse cytotoxic T cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat MKP-3 Monoclonal Antibody (Catalog # MAB3576) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MKP-3 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.