Explore
Validate
Learn
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [4]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00001848-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001848-M01, RRID:AB_538407
- Product name
- Mouse Monoclonal MKP-3/DUSP6 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. DUSP6 - dual specificity phosphatase 6
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references TSH signaling overcomes B-RafV600E-induced senescence in papillary thyroid carcinogenesis through regulation of DUSP6.
Molecular classification of nonsmall cell lung cancer using a 4-protein quantitative assay.
Dual specificity phosphatase 6 as a predictor of invasiveness in papillary thyroid cancer.
Down-regulation of DUSP6 expression in lung cancer: its mechanism and potential role in carcinogenesis.
Kim YH, Choi YW, Han JH, Lee J, Soh EY, Park SH, Kim JH, Park TJ
Neoplasia (New York, N.Y.) 2014 Dec;16(12):1107-20
Neoplasia (New York, N.Y.) 2014 Dec;16(12):1107-20
Molecular classification of nonsmall cell lung cancer using a 4-protein quantitative assay.
Anagnostou VK, Dimou AT, Botsis T, Killiam EJ, Gustavson MD, Homer RJ, Boffa D, Zolota V, Dougenis D, Tanoue L, Gettinger SN, Detterbeck FC, Syrigos KN, Bepler G, Rimm DL
Cancer 2012 Mar 15;118(6):1607-18
Cancer 2012 Mar 15;118(6):1607-18
Dual specificity phosphatase 6 as a predictor of invasiveness in papillary thyroid cancer.
Lee JU, Huang S, Lee MH, Lee SE, Ryu MJ, Kim SJ, Kim YK, Kim SY, Joung KH, Kim JM, Shong M, Jo YS
European journal of endocrinology 2012 Jul;167(1):93-101
European journal of endocrinology 2012 Jul;167(1):93-101
Down-regulation of DUSP6 expression in lung cancer: its mechanism and potential role in carcinogenesis.
Okudela K, Yazawa T, Woo T, Sakaeda M, Ishii J, Mitsui H, Shimoyamada H, Sato H, Tajiri M, Ogawa N, Masuda M, Takahashi T, Sugimura H, Kitamura H
The American journal of pathology 2009 Aug;175(2):867-81
The American journal of pathology 2009 Aug;175(2):867-81
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - DUSP6 monoclonal antibody (M01), clone 3G2. Western Blot analysis of DUSP6 expression in human placenta.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - DUSP6 monoclonal antibody (M01), clone 3G2 Western Blot analysis of DUSP6 expression in Jurkat ( Cat # L017V1 ).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - Analysis of DUSP6 over-expressed 293 cell line, cotransfected with DUSP6 Validated Chimera RNAi ( Cat # H00001848-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DUSP6 monoclonal antibody (M01), clone 3G2 (Cat # H00001848-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - Analysis of DUSP6 expression in transfected 293T cell line by DUSP6 monoclonal antibody (M01), clone 3G2.Lane 1: DUSP6 transfected lysate(42 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - Detection limit for recombinant GST tagged DUSP6 is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - Analysis of monoclonal antibody to DUSP6 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MKP-3/DUSP6 Antibody (3G2) [H00001848-M01] - Analysis of monoclonal antibody to DUSP6 on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml.
