LS-C817983

antibody from LSBio

Targeting: AIMP1 EMAP-2, EMAPII, p43, SCYE1

Western blot (Enhanced validation)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C817983

Provider

LSBio

Product name

AIMP1 / EMAP II Antibody (Preservative Free) LS-C817983

Antibody type

Polyclonal

Description

Protein A affinity chromatography

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-SCYE1 rabbit polyclonal antibody at 1:500 dilution. Lane A: H1299 Whole Cell Lysate. Lane B: HepG2 Whole Cell Lysate. Lane C: HEK293 Whole Cell Lysate. Lane D: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 38 kDa.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

SCYE1 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. 4 uL anti-SCYE1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-SCYE1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 35 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

SCYE1 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. 4 uL anti-SCYE1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-SCYE1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 35 kDa.