PA5-117774

antibody from Invitrogen Antibodies

Targeting: AIMP1 EMAP-2, EMAPII, p43, SCYE1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117774

Provider

Invitrogen Antibodies

Product name

EMAP II Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot of EMAP II in Lane A: H1299 Whole Cell Lysate, Lane B: HepG2 Whole Cell Lysate, Lane C: HEK293 Whole Cell Lysate, Lane D: Jurkat Whole Cell Lysate. Samples (30 µg per lane) were incubated with polyclonal antibody (Product # PA5-117774) with a dilution of 1:500 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 34 kDa, Observed band size: 38 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using EMAP II Polyclonal Antibody (Product # PA5-117774) at 1:500 dilution. Lane A: H1299 Whole Cell Lysate, Lane B: HepG2 Whole Cell Lysate, Lane C: HEK293 Whole Cell Lysate, Lane D: Jurkat Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 38 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining of SCYE1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with EMAP II Polyclonal Antibody (Product # PA5-117774, 1:500) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human EMAP II in human brain with EMAP II Polyclonal Antibody (Product # PA5-117774, 1:5,000 dilution, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human EMAP II in human pancreas with EMAP II Polyclonal Antibody (Product # PA5-117774, 1:5,000 dilution, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

EMAP II Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 4 µL with EMAP II Polyclonal Antibody (Product # PA5-117774) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: EMAP II Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 35 kDa. Observed band size: 35 kDa.