Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-21644 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EMAP II Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: H1299, HepG2.
- Concentration
- 0.54 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using EMAP II Polyclonal Antibody (Product # PA5-21644). Sample (30 µg of whole cell lysate). A: H1299. B: Hep G2. 12% SDS PAGE. EMAP II Polyclonal Antibody (Product # PA5-21644) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of EMAP II in paraformaldehyde-fixed HeLa cells using an EMAP II polyclonal antibody (Product # PA5-21644) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using AIMP1 antibody (Product # PA5-21644) (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Product # MA1-25054) (Red) at 1:2,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- EMAP II Polyclonal Antibody detects SCYE1 protein at cytosol on H1299 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded H1299 xenograft. EMAP II Polyclonal Antibody (Product # PA5-21644) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.