Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF1910 - Provider product page
- Provider
- R&D Systems
- Product name
- Human EMAP-II Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human and mouse EMAP-II in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q12904
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human EMAP-II by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and SK-OV-3 human ovarian adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human EMAP-II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1910) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EMAP-II at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human EMAP-II by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and OVCAR-3 human ovarian carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for EMAP-II at approximately 45 kDa (as indicated) using 10 µg/mL of Goat Anti-Human EMAP-II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1910) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- EMAP-II in Human Colon Cancer Tissue. EMAP-II was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Human EMAP-II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1910) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to epithelial and endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.