Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA501198 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501198, RRID:AB_11124745
- Product name
- Anti-PDE4A mouse monoclonal antibody, clone OTI3D4 (formerly 3D4)
- Antibody type
- Monoclonal
- Description
- Anti-PDE4A mouse monoclonal antibody, clone OTI3D4 (formerly 3D4)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PDE4A
- Isotype
- IgG
- Antibody clone number
- OTI3D4
- Vial size
- 100 µl
- Concentration
- 0.64 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE4A (RC207765, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE4A .
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-PDE4A mouse monoclonal antibody (TA501198) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE4A (RC207765 ).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HT29 cells using anti-PDE4A mouse monoclonal antibody (TA501198).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PDE4A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501198, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PDE4A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501198, Dilution 1:50)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-PDE4A antibody(TA501198),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
