HPA052818

antibody from Atlas Antibodies

Targeting: MED1 CRSP1, CRSP200, DRIP230, PBP, PPARBP, PPARGBP, RB18A, TRAP220, TRIP2

Immunocytochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA052818

Provider

Atlas Antibodies

Proper citation

Product name

Anti-MED1

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human MED1, Gene description: mediator complex subunit 1, Alternative Gene Names: CRSP1, CRSP200, DRIP230, PBP, PPARBP, PPARGBP, RB18A, TRAP220, TRIP2, Validated applications: ICC, ChIP, Uniprot ID: Q15648, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.1 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

Notch1 Phase Separation Coupled Percolation facilitates target gene expression and enhancer looping.

Foran G, Hallam RD, Megaly M, Turgambayeva A, Antfolk D, Li Y, Luca VC, Necakov A
bioRxiv : the preprint server for biology 2024 Aug 1;

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Regulated splicing of large exons is linked to phase‐separation of vertebrate transcription factors

Kawachi T, Masuda A, Yamashita Y, Takeda J, Ohkawara B, Ito M, Ohno K
The EMBO Journal 2021;40(22)

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Sample type

Human

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

ChIP-Exo-Seq composite graph for Anti-MED1 (HPA052818, Lot 000011173) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.