CABT-B9202

antibody from Creative Diagnostics

Targeting: TCF3 bHLHb21, E2A, E47, ITF1, MGC129647, MGC129648, p75, VDIR

Western blot (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Gel shift (Recommended by provider)

Antibody Data

Product number

CABT-B9202

Provider

Creative Diagnostics

Product name

Mouse anti-Human E12/E47 monoclonal antibody, clone H09-382

Antibody type

Monoclonal

Description

B lymphoid cells go through many intermediate steps before becoming plasma ells whichproduce and secrete immunoglobulins (Ig). The earliest stage of B-cell differentiate is represented by pro-B lymphocytes that contain both the Ig heavy- and light-chain genes in the transcriptionally inactive germ line cnfiguration. As ro-B lymphocytes differentiate into pro-B cells, Ig heavy-chain genes rearrange and get transcribed and translated. However, the Ig lightchain genes are not transcribed before the pre-B cells differentiate into mature B-lymphocytes. The developmental regulation of the Ig gene expression is dependent on various sequences in the Ig enhancer region. One class of such regulatory sequence elements comprises the so-called E-boxes which share the NNCANNTGNN consensus sequence. The E2 boxes are particularly interesting because they are present in muscle and pancreas-specific enhancers. A family of proteins binds to the E2 box. These proteins share a common amino acid sequence motif that is proposed to form two amphipathic helices interrupted by a loop, designated the helix-loop-helix (HLH) motif. The HLH motif mediates homo- as well as heterodimerization with other HLH proteins. Most HLH proteins possess a basic region located N terminal of the HLH region which is responsible for DNA binding. Two E2 box binding proteins have been described (E12 and E47) that arise as alternatively spliced form of the E2A gene. E12 and E47 are identical except in the HLH region. These two proteins have been directly implicated in the regulation of B-cell, muscle and pancreas-specific gene expression. The E2-2 gene product is closely related to the E2A gene products but is encoded by a separate gene. E47 migrates at a reduced molecular weight of ~75 kDa. G98-271 reacts specifically with human E12 and E47 protein, alternatively spliced products of the E2A gene. It does not cross-react with the E2-2 protein. The specificity of the antibody was tested by immunoprecipitation and electrophoretic mobility band shift assays (EMSA) using recombinant protein (E2-2, E12, and E4 7). The HLH motif as well as flanking regions of recombinant human E12 potein was used as the immunogen.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

H09-382

Vial size

0.1mg

Concentration

0.5 mg/ml

Storage

Store undiluted at -20°C.