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LearnMAB44061
antibody from R&D Systems
Targeting: TCF3
bHLHb21, E2A, E47, ITF1, MGC129647, MGC129648, p75, VDIR
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- MAB44061 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TCF-3/E2A Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human TCF-3/E2A in direct ELISAs. In this format, 100% cross-reactivity with recombinant human (rh) TCF-12 is observed and no cross-reactivity with rhTCF-1, 2, 4, 7L1, 7L2 or rhLEF-1 is observed.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
NP_003191- Isotype
- IgG
- Antibody clone number
- 401709
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references MicroRNA-449a maintains self-renewal in liver cancer stem-like cells by targeting Tcf3.
Zhang Q, Yang Z, Shan J, Liu L, Liu C, Shen J, Chen X, Xu Y, Chen J, Ma Q, Yang L, Qian C
Oncotarget 2017 Dec 15;8(66):110187-110200
Oncotarget 2017 Dec 15;8(66):110187-110200
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TCF-3/E2A in HeLa Human Cell Line. TCF-3/E2A was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Human TCF-3/E2A Monoclonal Antibody (Catalog # MAB44061) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nucleoli. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
